Characterization of the Role Rab25 in Energy Metabolism and Cancer Using Extracellular Flux Analysis and Material Balance View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2015

AUTHORS

Shreya Mitra , Jennifer Molina , Gordon B. Mills , Jennifer B. Dennison

ABSTRACT

Rab25, by altering trafficking of critical cellular resources, influences cell metabolism and survival during stress conditions. Overall, perturbations in the vesicular trafficking machinery change cellular bioenergetics that can be directly measured in real time as Oxygen Consumption Rate, OCR (mitochondrial respiration) and Extracellular Acidification Rate, ECAR (glycolysis) by an extracellular flux analyzer (XF96, Seahorse Biosciences, MA). Additionally, overall turnover of glucose, lactate, as well as glutamine and glutamate can be measured biochemically using the YSI2900 Biochemistry Analyzer (YSI Incorporated, Life Sciences, OH). A combination of these two methods allows a precise and quantitative approach to interrogate the role of Rab25 as well as other Rab GTPases in central carbon energy metabolism. More... »

PAGES

195-205

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-2569-8_17

DOI

http://dx.doi.org/10.1007/978-1-4939-2569-8_17

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1009589205

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/25800844


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