Characterizing the DNA Binding Site Specificity of NF-κB with Protein-Binding Microarrays (PBMs) View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2015

AUTHORS

Trevor Siggers , Thomas D. Gilmore , Brian Barron , Ashley Penvose

ABSTRACT

NF-κB transcription factors control a wide array of important cellular and organismal processes in eukaryotes. All NF-κB transcription factors bind to DNA target sites as dimers. In vertebrates, there are five NF-κB subunits, p50, p52, RelA (p65), c-Rel, and RelB, that can form almost all combinations of homodimers and heterodimers, which recognize distinct, but overlapping, target sequences. In this chapter, we describe the use of protein-binding microarrays (PBMs), a high-throughput method to measure the binding of proteins to different DNA sequences. PBM datasets allow for sensitive comparisons of NF-κB dimer DNA-binding differences and can aid in the computational and experimental prediction of NF-κB target genes. More... »

PAGES

609-30

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-2422-6_36

DOI

http://dx.doi.org/10.1007/978-1-4939-2422-6_36

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1016612990

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/25736775


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