Ontology type: schema:Chapter
2015
AUTHORSGeorges Pelletier , Francoise Budar
ABSTRACTThis chapter retraces the history of using somatic hybridization to modify the cytoplasmic male sterility (CMS)-inducing Ogura radish cytoplasm and its application in hybrid seed production for Brassica crops. First, we present hybrid seed production and the issues behind the introduction of the Ogura radish cytoplasm into Brassica crops. We then detail the procedures of the protoplast fusion experiments used to produce cybrids. Recombination between radish and Brassica mitochondrial genomes ultimately led to the creation of the Ogu-INRA system. Not only have cybrids contributed to hybrid seed production but also molecular analysis of cybrids helped identify the mitochondrial gene causing CMS, and contributed basic knowledge on plant mitochondrial gene expression. Furthermore, restoration of male fertility is useful for some crops, and the Ogu-INRA system has also been used to identify restorer genes. The Ogu-INRA system is now widely used in agriculture. More... »
PAGES199-216
Somatic Genome Manipulation
ISBN
978-1-4939-2388-5
978-1-4939-2389-2
http://scigraph.springernature.com/pub.10.1007/978-1-4939-2389-2_9
DOIhttp://dx.doi.org/10.1007/978-1-4939-2389-2_9
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