Sex Identification of Ancient DNA Samples Using a Microfluidic Device View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2015

AUTHORS

Kirsty J. Shaw , Keri A. Brown , Terence A. Brown , Stephen J. Haswell

ABSTRACT

Ancient DNA is the name given to the degraded, fragmented, and chemically damaged biomolecules that can be recovered from archaeological remains of plants, animals, and humans. Where ancient human DNA has survived at archaeological sites, it can give valuable information and is especially useful for its potential to identify kinship, population affinities, pathogens, and biological sex. Here, we describe the operation of a microfluidic device for the sex identification of ancient DNA samples using an efficient sample handling process. DNA is extracted from powdered bone samples and abasic sites labeled with biotin. Streptavidin-coated superparamagnetic particles are used to isolate the labeled DNA prior to amplification of the Amelogenin sex marker. More... »

PAGES

93-8

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-2353-3_8

DOI

http://dx.doi.org/10.1007/978-1-4939-2353-3_8

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1034461945

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/25673485


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