Measurement of Mitochondrial NADH and FAD Autofluorescence in Live Cells View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2015

AUTHORS

Fernando Bartolomé , Andrey Y. Abramov

ABSTRACT

In the process of energy production, mitochondrial networks are key elements to allow metabolism of substrates into ATP. Many pathological conditions have been associated with mitochondrial dysfunction as mitochondria are associated with a wide range of cellular processes. Therefore, any disruption in the energy production induces devastating effects that can ultimately lead to cell death due to chemical ischemia. To address the mitochondrial health and function, there are several bioenergetic parameters reflecting either whole mitochondrial functionality or individual mitochondrial complexes. Particularly, metabolism of nutrients in the tricarboxylic acid cycle provides substrates used to generate electron carriers (nicotinamide adenine dinucleotide [NADH] and flavin adenine dinucleotide [FADH2]) which ultimately donate electrons to the mitochondrial electron transport chain. The levels of NADH and FADH2 can be estimated through imaging of NADH/NAD(P)H or FAD autofluorescence. This report demonstrates how to perform and analyze NADH/NAD(P)H and FAD autofluorescence in a time-course-dependent manner and provides information about NADH and FAD redox indexes both reflecting the activity of the mitochondrial electron transport chain (ETC). Furthermore, total pools of NADH and FAD can be estimated providing information about the rate of substrate supply into the ETC. Finally, the analysis of NADH autofluorescence after induction of maximal respiration can offer information about the pentose phosphate pathway activity where glucose can be alternatively oxidized instead of pyruvate. More... »

PAGES

263-70

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-2257-4_23

DOI

http://dx.doi.org/10.1007/978-1-4939-2257-4_23

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1011063028

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/25631020


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Flavin-Adenine Dinucleotide", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fluorometry", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mitochondria", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Imaging", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "NAD", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "name": [
            "Neurosciences group, Instituto de Investigacion Hospital 12 de Octubre (i+12), Av Cordoba, Madrid, 28041, Spain"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bartolom\u00e9", 
        "givenName": "Fernando", 
        "id": "sg:person.0716241731.24", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0716241731.24"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Neurosciences group, Instituto de Investigacion Hospital 12 de Octubre (i+12), Av Cordoba, Madrid, 28041, Spain"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Abramov", 
        "givenName": "Andrey Y.", 
        "id": "sg:person.01162011564.28", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01162011564.28"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1042/bj20110162", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001293861"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj20110162", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001293861"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature05292", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003167107", 
          "https://doi.org/10.1038/nature05292"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature05292", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003167107", 
          "https://doi.org/10.1038/nature05292"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature05292", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003167107", 
          "https://doi.org/10.1038/nature05292"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.mito.2013.03.009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021365478"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.neuron.2013.02.028", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030229153"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1471-4159.2006.03988.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041387445"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2013.05.039", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041626640"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1517/14728221003730434", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044024803"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1155/2012/428010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051805402"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1517/14728221003652489", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052739438"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj20130863", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056716572"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj20130863", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056716572"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2741/2132", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1070914440"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2015", 
    "datePublishedReg": "2015-01-01", 
    "description": "In the process of energy production, mitochondrial networks are key elements to allow metabolism of substrates into ATP. Many pathological conditions have been associated with mitochondrial dysfunction as mitochondria are associated with a wide range of cellular processes. Therefore, any disruption in the energy production induces devastating effects that can ultimately lead to cell death due to chemical ischemia. To address the mitochondrial health and function, there are several bioenergetic parameters reflecting either whole mitochondrial functionality or individual mitochondrial complexes. Particularly, metabolism of nutrients in the tricarboxylic acid cycle provides substrates used to generate electron carriers (nicotinamide adenine dinucleotide [NADH] and flavin adenine dinucleotide [FADH2]) which ultimately donate electrons to the mitochondrial electron transport chain. The levels of NADH and FADH2 can be estimated through imaging of NADH/NAD(P)H or FAD autofluorescence. This report demonstrates how to perform and analyze NADH/NAD(P)H and FAD autofluorescence in a time-course-dependent manner and provides information about NADH and FAD redox indexes both reflecting the activity of the mitochondrial electron transport chain (ETC). Furthermore, total pools of NADH and FAD can be estimated providing information about the rate of substrate supply into the ETC. Finally, the analysis of NADH autofluorescence after induction of maximal respiration can offer information about the pentose phosphate pathway activity where glucose can be alternatively oxidized instead of pyruvate. ", 
    "editor": [
      {
        "familyName": "Weissig", 
        "givenName": "Volkmar", 
        "type": "Person"
      }, 
      {
        "familyName": "Edeas", 
        "givenName": "Marvin", 
        "type": "Person"
      }
    ], 
    "genre": "chapter", 
    "id": "sg:pub.10.1007/978-1-4939-2257-4_23", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": {
      "isbn": [
        "978-1-4939-2256-7", 
        "978-1-4939-2257-4"
      ], 
      "name": "Mitochondrial Medicine", 
      "type": "Book"
    }, 
    "name": "Measurement of Mitochondrial NADH and FAD Autofluorescence in Live Cells", 
    "pagination": "263-70", 
    "productId": [
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/978-1-4939-2257-4_23"
        ]
      }, 
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "34b56b578826fc93834c9ab9b17ea8612b0285ebc822931556aa13869e567272"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1011063028"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "25631020"
        ]
      }
    ], 
    "publisher": {
      "location": "New York, NY", 
      "name": "Springer New York", 
      "type": "Organisation"
    }, 
    "sameAs": [
      "https://doi.org/10.1007/978-1-4939-2257-4_23", 
      "https://app.dimensions.ai/details/publication/pub.1011063028"
    ], 
    "sdDataset": "chapters", 
    "sdDatePublished": "2019-04-15T12:30", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8663_00000250.jsonl", 
    "type": "Chapter", 
    "url": "http://link.springer.com/10.1007/978-1-4939-2257-4_23"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/978-1-4939-2257-4_23'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/978-1-4939-2257-4_23'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/978-1-4939-2257-4_23'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/978-1-4939-2257-4_23'


 

This table displays all metadata directly associated to this object as RDF triples.

144 TRIPLES      23 PREDICATES      46 URIs      28 LITERALS      16 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/978-1-4939-2257-4_23 schema:about N0bd45bcb02a546b2a2eade6b0e627fb2
2 N1f8d845d9d244ecdac734c738154d3f6
3 N607c099fa2524f628f2d0a18f93fe67a
4 N682c5cbcc6144fae81b390bbf0251a03
5 N86ab4adf77d94dc89ce89a0ab9678610
6 N9ec81a05639743fe985052a6f0ff56f9
7 Nd995b6fcb2be4ebd881bbd05538ea1db
8 anzsrc-for:06
9 anzsrc-for:0601
10 schema:author N3a65097c27ad4d33b1e3b8af9a347194
11 schema:citation sg:pub.10.1038/nature05292
12 https://doi.org/10.1016/j.cell.2013.05.039
13 https://doi.org/10.1016/j.mito.2013.03.009
14 https://doi.org/10.1016/j.neuron.2013.02.028
15 https://doi.org/10.1042/bj20110162
16 https://doi.org/10.1042/bj20130863
17 https://doi.org/10.1111/j.1471-4159.2006.03988.x
18 https://doi.org/10.1155/2012/428010
19 https://doi.org/10.1517/14728221003652489
20 https://doi.org/10.1517/14728221003730434
21 https://doi.org/10.2741/2132
22 schema:datePublished 2015
23 schema:datePublishedReg 2015-01-01
24 schema:description In the process of energy production, mitochondrial networks are key elements to allow metabolism of substrates into ATP. Many pathological conditions have been associated with mitochondrial dysfunction as mitochondria are associated with a wide range of cellular processes. Therefore, any disruption in the energy production induces devastating effects that can ultimately lead to cell death due to chemical ischemia. To address the mitochondrial health and function, there are several bioenergetic parameters reflecting either whole mitochondrial functionality or individual mitochondrial complexes. Particularly, metabolism of nutrients in the tricarboxylic acid cycle provides substrates used to generate electron carriers (nicotinamide adenine dinucleotide [NADH] and flavin adenine dinucleotide [FADH2]) which ultimately donate electrons to the mitochondrial electron transport chain. The levels of NADH and FADH2 can be estimated through imaging of NADH/NAD(P)H or FAD autofluorescence. This report demonstrates how to perform and analyze NADH/NAD(P)H and FAD autofluorescence in a time-course-dependent manner and provides information about NADH and FAD redox indexes both reflecting the activity of the mitochondrial electron transport chain (ETC). Furthermore, total pools of NADH and FAD can be estimated providing information about the rate of substrate supply into the ETC. Finally, the analysis of NADH autofluorescence after induction of maximal respiration can offer information about the pentose phosphate pathway activity where glucose can be alternatively oxidized instead of pyruvate.
25 schema:editor Ne94c43a21ce34641ba9ac5824ba5fe92
26 schema:genre chapter
27 schema:inLanguage en
28 schema:isAccessibleForFree false
29 schema:isPartOf Ncb56079c59ac41c2b21221880db517df
30 schema:name Measurement of Mitochondrial NADH and FAD Autofluorescence in Live Cells
31 schema:pagination 263-70
32 schema:productId N2df22e10b1c148dc818a99f8d52b56f3
33 N4e6fa33573bc4e13ab0f7dfb7eb888f4
34 Na577c22d0b8046269c6b4179a2a15973
35 Nbb6152f89b62410f92d33022a537ee9a
36 schema:publisher N8e9a8b3cec514837b3f19ad975eb822e
37 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011063028
38 https://doi.org/10.1007/978-1-4939-2257-4_23
39 schema:sdDatePublished 2019-04-15T12:30
40 schema:sdLicense https://scigraph.springernature.com/explorer/license/
41 schema:sdPublisher N6502028ad1b240faad2f58909dfaa724
42 schema:url http://link.springer.com/10.1007/978-1-4939-2257-4_23
43 sgo:license sg:explorer/license/
44 sgo:sdDataset chapters
45 rdf:type schema:Chapter
46 N0bd45bcb02a546b2a2eade6b0e627fb2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
47 schema:name Flavin-Adenine Dinucleotide
48 rdf:type schema:DefinedTerm
49 N1f8d845d9d244ecdac734c738154d3f6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
50 schema:name Mitochondria
51 rdf:type schema:DefinedTerm
52 N2df22e10b1c148dc818a99f8d52b56f3 schema:name pubmed_id
53 schema:value 25631020
54 rdf:type schema:PropertyValue
55 N3a65097c27ad4d33b1e3b8af9a347194 rdf:first sg:person.0716241731.24
56 rdf:rest Nd557db191c404a2baa81f0b71c0c5920
57 N4e6fa33573bc4e13ab0f7dfb7eb888f4 schema:name readcube_id
58 schema:value 34b56b578826fc93834c9ab9b17ea8612b0285ebc822931556aa13869e567272
59 rdf:type schema:PropertyValue
60 N607c099fa2524f628f2d0a18f93fe67a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
61 schema:name Molecular Imaging
62 rdf:type schema:DefinedTerm
63 N6502028ad1b240faad2f58909dfaa724 schema:name Springer Nature - SN SciGraph project
64 rdf:type schema:Organization
65 N682c5cbcc6144fae81b390bbf0251a03 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
66 schema:name Fluorometry
67 rdf:type schema:DefinedTerm
68 N86ab4adf77d94dc89ce89a0ab9678610 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
69 schema:name NAD
70 rdf:type schema:DefinedTerm
71 N892260a8f062489c8b4354d57f30c369 schema:familyName Edeas
72 schema:givenName Marvin
73 rdf:type schema:Person
74 N8e9a8b3cec514837b3f19ad975eb822e schema:location New York, NY
75 schema:name Springer New York
76 rdf:type schema:Organisation
77 N9ec81a05639743fe985052a6f0ff56f9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
78 schema:name Humans
79 rdf:type schema:DefinedTerm
80 Na577c22d0b8046269c6b4179a2a15973 schema:name dimensions_id
81 schema:value pub.1011063028
82 rdf:type schema:PropertyValue
83 Na7b0131bf24c4a7bb05b4667812333cb schema:familyName Weissig
84 schema:givenName Volkmar
85 rdf:type schema:Person
86 Nbb6152f89b62410f92d33022a537ee9a schema:name doi
87 schema:value 10.1007/978-1-4939-2257-4_23
88 rdf:type schema:PropertyValue
89 Ncb56079c59ac41c2b21221880db517df schema:isbn 978-1-4939-2256-7
90 978-1-4939-2257-4
91 schema:name Mitochondrial Medicine
92 rdf:type schema:Book
93 Nd0d592b091f24f7d84e0b6c7fd823aeb schema:name Neurosciences group, Instituto de Investigacion Hospital 12 de Octubre (i+12), Av Cordoba, Madrid, 28041, Spain
94 rdf:type schema:Organization
95 Nd1f0bf964c5849649325377406bad293 schema:name Neurosciences group, Instituto de Investigacion Hospital 12 de Octubre (i+12), Av Cordoba, Madrid, 28041, Spain
96 rdf:type schema:Organization
97 Nd557db191c404a2baa81f0b71c0c5920 rdf:first sg:person.01162011564.28
98 rdf:rest rdf:nil
99 Nd995b6fcb2be4ebd881bbd05538ea1db schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
100 schema:name Cell Line
101 rdf:type schema:DefinedTerm
102 Ne32cf59d98c344faaab23f90cc1ee07d rdf:first N892260a8f062489c8b4354d57f30c369
103 rdf:rest rdf:nil
104 Ne94c43a21ce34641ba9ac5824ba5fe92 rdf:first Na7b0131bf24c4a7bb05b4667812333cb
105 rdf:rest Ne32cf59d98c344faaab23f90cc1ee07d
106 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
107 schema:name Biological Sciences
108 rdf:type schema:DefinedTerm
109 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
110 schema:name Biochemistry and Cell Biology
111 rdf:type schema:DefinedTerm
112 sg:person.01162011564.28 schema:affiliation Nd1f0bf964c5849649325377406bad293
113 schema:familyName Abramov
114 schema:givenName Andrey Y.
115 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01162011564.28
116 rdf:type schema:Person
117 sg:person.0716241731.24 schema:affiliation Nd0d592b091f24f7d84e0b6c7fd823aeb
118 schema:familyName Bartolomé
119 schema:givenName Fernando
120 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0716241731.24
121 rdf:type schema:Person
122 sg:pub.10.1038/nature05292 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003167107
123 https://doi.org/10.1038/nature05292
124 rdf:type schema:CreativeWork
125 https://doi.org/10.1016/j.cell.2013.05.039 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041626640
126 rdf:type schema:CreativeWork
127 https://doi.org/10.1016/j.mito.2013.03.009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021365478
128 rdf:type schema:CreativeWork
129 https://doi.org/10.1016/j.neuron.2013.02.028 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030229153
130 rdf:type schema:CreativeWork
131 https://doi.org/10.1042/bj20110162 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001293861
132 rdf:type schema:CreativeWork
133 https://doi.org/10.1042/bj20130863 schema:sameAs https://app.dimensions.ai/details/publication/pub.1056716572
134 rdf:type schema:CreativeWork
135 https://doi.org/10.1111/j.1471-4159.2006.03988.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1041387445
136 rdf:type schema:CreativeWork
137 https://doi.org/10.1155/2012/428010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051805402
138 rdf:type schema:CreativeWork
139 https://doi.org/10.1517/14728221003652489 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052739438
140 rdf:type schema:CreativeWork
141 https://doi.org/10.1517/14728221003730434 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044024803
142 rdf:type schema:CreativeWork
143 https://doi.org/10.2741/2132 schema:sameAs https://app.dimensions.ai/details/publication/pub.1070914440
144 rdf:type schema:CreativeWork
 




Preview window. Press ESC to close (or click here)


...