Time-Resolved Spectrometry of Mitochondrial NAD(P)H Fluorescence and Its Applications for Evaluating the Oxidative State in Living Cells View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2015

AUTHORS

Julia Horilova , Hauke Studier , Zuzana Nadova , Pavol Miskovsky , Dusan Chorvat , Alzbeta Marcek Chorvatova

ABSTRACT

Time-resolved fluorescence spectrometry is a highly valuable technological tool to detect and characterize mitochondrial metabolic oxidative changes by means of endogenous fluorescence (Chorvat and Chorvatova, Laser Phys Lett 6: 175-193, 2009). Here, we describe the detection and measurement of endogenous mitochondrial NAD(P)H (nicotinamide adenine dinucleotide (phosphate)) fluorescence directly in living cultured cells using fluorescence lifetime spectrometry imaging after excitation with 405 nm picosecond (ps) laser. Time-correlated single photon counting (TCSPC) method is employed. More... »

PAGES

183-93

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-2257-4_17

DOI

http://dx.doi.org/10.1007/978-1-4939-2257-4_17

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1042745126

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/25631014


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44 schema:description Time-resolved fluorescence spectrometry is a highly valuable technological tool to detect and characterize mitochondrial metabolic oxidative changes by means of endogenous fluorescence (Chorvat and Chorvatova, Laser Phys Lett 6: 175-193, 2009). Here, we describe the detection and measurement of endogenous mitochondrial NAD(P)H (nicotinamide adenine dinucleotide (phosphate)) fluorescence directly in living cultured cells using fluorescence lifetime spectrometry imaging after excitation with 405 nm picosecond (ps) laser. Time-correlated single photon counting (TCSPC) method is employed.
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