BAC Sequencing Using Pooled Methods View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2014-09-04

AUTHORS

Christopher A. Saski , F. Alex Feltus , Laxmi Parida , Niina Haiminen

ABSTRACT

Shotgun sequencing and assembly of a large, complex genome can be both expensive and challenging to accurately reconstruct the true genome sequence. Repetitive DNA arrays, paralogous sequences, polyploidy, and heterozygosity are main factors that plague de novo genome sequencing projects that typically result in highly fragmented assemblies and are difficult to extract biological meaning. Targeted, sub-genomic sequencing offers complexity reduction by removing distal segments of the genome and a systematic mechanism for exploring prioritized genomic content through BAC sequencing. If one isolates and sequences the genome fraction that encodes the relevant biological information, then it is possible to reduce overall sequencing costs and efforts that target a genomic segment. This chapter describes the sub-genome assembly protocol for an organism based upon a BAC tiling path derived from a genome-scale physical map or from fine mapping using BACs to target sub-genomic regions. Methods that are described include BAC isolation and mapping, DNA sequencing, and sequence assembly. More... »

PAGES

55-67

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-1652-8_3

DOI

http://dx.doi.org/10.1007/978-1-4939-1652-8_3

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1010935718

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/25239741


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