Metabolomic and Lipidomic Analyses of Chronologically Aging Yeast View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2014-08-12

AUTHORS

Vincent R. Richard , Simon D. Bourque , Vladimir I. Titorenko

ABSTRACT

Metabolomic and lipidomic analyses of yeast cells provide comprehensive empirical datasets for unveiling mechanisms underlying complex biological processes. In this chapter, we describe detailed protocols for using such analyses to study the age-related dynamics of changes in intracellular and extracellular levels of various metabolites and membrane lipids in chronologically aging yeast. The protocols for the following high-throughput analyses are described: (1) microanalytic biochemical assays for monitoring intracellular concentrations of trehalose and glycogen; (2) gas chromatographic quantitative assessment of extracellular concentrations of ethanol and acetic acid; and (3) mass spectrometric identification and quantitation of the entire complement of cellular lipids. These protocols are applicable to the exploration of the metabolic patterns associated not only with aging but also with many other vital processes in yeast. The described here methodology complements the powerful genetic approaches available for mechanistic studies of fundamental aspects of yeast biology. More... »

PAGES

359-373

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-1363-3_21

DOI

http://dx.doi.org/10.1007/978-1-4939-1363-3_21

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1016309865

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/25213255


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