Viral Nucleic Acid Recognition in Human Nonimmune Cells: In Vitro Systems View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2014

AUTHORS

Andrea Ribeiro , Markus Wörnle

ABSTRACT

The innate immune system detects viral infections through a variety of receptors that sense the virus via their nucleic acid genome and evokes initial antiviral responses. Nonimmune cells possess pathogen-recognition mechanisms that enable them to respond to virus by the expression of RNA or DNA recognition receptors, thereby initiating the first steps in the host-pathogen interaction and inducing the production of pro-inflammatory and antiviral mediators. To better understand the activation of innate-immune defense mechanisms by nonimmune cells, we describe an experimental procedure that mimics viral infection, using four human nonimmune cell culture models under stimulation with synthetic analogues of RNA and DNA virus. Furthermore, we exemplify two viral infection models using cultured nonimmune cells and hepatitis C virus (HCV) or polyomavirus BK (BKV). In addition, we report the importance of siRNA technique in gene regulation in order to identify specific markers involved in innate antiviral responses in these cells. More... »

PAGES

67-75

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-0882-0_7

DOI

http://dx.doi.org/10.1007/978-1-4939-0882-0_7

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1036953006

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24957230


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