Generation of Nonpolar Deletion Mutants in Listeria monocytogenes Using the “SOEing” Method View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2014-04-11

AUTHORS

Kathrin Rychli , Caitriona M. Guinane , Karen Daly , Colin Hill , Paul D. Cotter

ABSTRACT

The ability to manipulate chromosomally encoded genes is a fundamental biological tool for the analysis of gene function. Here, we provide in greater depth protocols for the creation of nonpolar unlabeled gene deletions in Listeria (L.) monocytogenes that are facilitated by the splicing overlap extension PCR technique. For mutagenesis in L. monocytogenes, we describe two different plasmid-based approaches, which facilitate the introduction of this spliced amplicon in place of the corresponding segment of chromosomal DNA: the pKSV7 system and the pORI280/pVE6007 system. More... »

PAGES

187-200

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-0703-8_16

DOI

http://dx.doi.org/10.1007/978-1-4939-0703-8_16

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1038691552

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24792559


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