Generation of Multiple Peptide Cocktail-Pulsed Dendritic Cells as a Cancer Vaccine View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2014

AUTHORS

Hyun-Ju Lee , Nu-Ri Choi , Manh-Cuong Vo , My-Dung Hoang , Youn-Kyung Lee , Je-Jung Lee

ABSTRACT

Cancer immunotherapy based on dendritic cell (DC) vaccination has promising alternatives for the treatment of cancer. A central tenet of DC-based cancer immunotherapy is the generation of antigen-specific cytotoxic T lymphocyte (CTL) response. Tumor-associated antigens (TAA) and DC play pivotal roles in this process. DCs are well known to be the most potent antigen-presenting cells and have the most powerful antigen-presenting capacity. DCs pulsed with various TAA have been shown to be effective in producing specific antitumor effects both in vitro and in vivo. Several types of tumor antigens have been applied in cancer treatment including tumor RNA, lysates, apoptotic bodies, heat shock protein, peptides from TAA, and allogeneic tumor cells. Among them, the use of immunogenic HLA-A*0201-specific epitopes from multiple TAA enhances induction of antigen-specific CTL and associated therapeutic efficacy in HLA-A*0201(+) cancer patients. The current chapter provides a detailed protocol of generating multiple peptide cocktail-pulsed DC to elicit CTL with a broad spectrum of immune responses against the related tumor antigens. More... »

PAGES

17-26

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-0345-0_2

DOI

http://dx.doi.org/10.1007/978-1-4939-0345-0_2

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1051508876

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24619666


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