Lipid Raft Proteins and Their Identification in T Lymphocytes View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2004

AUTHORS

Bernd Wollscheid , Priska D. von Haller , Eugene Yi , Samuel Donohoe , Kelly Vaughn , Andrew Keller , Alexey I. Nesvizhskii , Jimmy Eng , Xiao-jun Li , David R. Goodlett , Ruedi Aebersold , Julian D. Watts

ABSTRACT

This review focuses on how membrane lipid rafts have been detected and isolated, mostly from lymphocytes, and their associated proteins identified. These proteins include transmembrane antigens/receptors, GPI-anchored proteins, cytoskeletal proteins, Src-family protein kinases, G-proteins, and other proteins involved in signal transduction. To further understand the biology of lipid rafts, new methodological approaches are needed to help characterize the raft protein component, and changes that occur in this component as a result of cell perturbation. We describe the application of new proteomic approaches to the identification and quantification of raft proteins in T-lymphocytes. Similar approaches, applied to other model cell systems, will provide valuable new insights into both cellular signal transduction and lipid raft biology. More... »

PAGES

121-152

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4757-5806-1_3

DOI

http://dx.doi.org/10.1007/978-1-4757-5806-1_3

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1049205529

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/15376619


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