The effect of intracellular proteinases on transformation of human lymphocytes. View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1986

AUTHORS

Mladen Korbelik , Janez Škrk , Primož Schauer , Alojz Suhar , Vito Turk , Miha Likar

ABSTRACT

The effect of two intracellular proteinases, calf liver chymotrypsin-like neutral proteinase and human brain cathepsin H, and of two proteinase inhibitors, leupeptin and endogenous cysteine proteinases inhibitor, was studied on unstimulated and mitogen-stimulated peripheral human blood lymphocytes. Classification of blood samples into four types, on the basis of their potential for spontaneous and mitogen-stimulated transformation, helped in analyzing the results. Within some of these types, at least, there appears certain uniformity of response to proteinase action. Lymphocytes most susceptible to proteinase action were those that were insensitive to phytohemagglutinin mitogenic stimulus. The maximal effect of proteinases or their inhibitors was obtained most often when these agents were added to lymphocyte cultures 24 hours before mitogen. Both proteinases, although of quite different specificities, gave very often results of similar trend on the same lymphocyte culture. Moreover, this was followed also by the two inhibitors, which showed not only inhibitory, but also potentiating power of action on lymphocyte transformation. The regulatory nature of the action of proteinases within lymphocytes and other cells is underlined. More... »

PAGES

113-20

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4757-0154-8_14

DOI

http://dx.doi.org/10.1007/978-1-4757-0154-8_14

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1079812947

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/3544722


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