Comparative Studies on tRNAs and Aminoacyl-tRNA Synthetases from Various Photosynthetic Organisms View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1983

AUTHORS

J. H. Weil , M. Mubumbila , M. Kuntz , M. Keller , E. J. Crouse , G. Burkard , P. Guillemaut , R. Selden , L. McIntosh , L. Bogorad , W. Löffelhardt , H. Mucke , H. J. Bohnert , A. Dietrich , G. Souciet , B. Colas , P. Imbault , V. Sarantoglou

ABSTRACT

Chloroplasts have their own protein synthesizing system, using chloroplast-specific tRNAs which are different from their cytoplasmic counterparts and are coded for by chloroplast DNA (Weil, 1979; Weil and Parthier, 1982). Total chloroplast tRNA can be fractionated by two-dimensional polyacrylamide gel electrophoresis (Burkard et al., 1982) into individual tRNAs, which can be recovered from the gel, identified by aminoacylation and labeled with 32P at their 3′ end using α-32P-ATP and tRNA nucleotidyl transferase (Mubumbila et al., 1980). Each labeled tRNA can then be hybridized to DNA fragments which have been generated by the action of a restriction endonuclease on chloroplast DNA, fractionated by agarose gel electrophoresis, and transferred to nitrocellulose strips. As the position of these fragments on the circular map of the chloroplast chromosome has been previously determined, this approach allows the localization of the tRNA genes. Such a tRNA gene map was first established in the case of the spinach chloroplast genome (Driesel et al., 1979), which is a circular molecule containing two inverted repeats; each of these inverted repeats contains a set of ribosomal RNA genes, and the spacer located between the 16S and the 23S rRNA gene was shown to contain a tRNAIle gene (Bohnert et al., 1979). Such tRNA gene mapping studies have now been extended to the chloroplast genomes of other photosynthetic organisms. More... »

PAGES

167-180

Book

TITLE

Structure and Function of Plant Genomes

ISBN

978-1-4684-4540-4
978-1-4684-4538-1

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4684-4538-1_15

DOI

http://dx.doi.org/10.1007/978-1-4684-4538-1_15

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1011053845


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Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/978-1-4684-4538-1_15'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/978-1-4684-4538-1_15'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/978-1-4684-4538-1_15'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/978-1-4684-4538-1_15'


 

This table displays all metadata directly associated to this object as RDF triples.

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