Time Course of Electropermeabilization View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1992

AUTHORS

J. Teissie

ABSTRACT

Cell membrane selective permeability is an advantage by preserving the genome and the cellular machinery from the action of exogenous agression but in the same time is a strong limitation for the experimental manipulation of the cytoplasm. Spontaneous introduction of foreign proteins or genes is not possible and artificial methods must be found which of course should alter the membrane permeability but not affect the viability. Electropulsation, i.e. submitting cells to strong shortlived electric field pulses, is a new methodology, which is now proved to be highly efficient to give access to the cytoplasm. Electropermeabilization and electrotransformation are very potent tools for bioengineering. More... »

PAGES

285-301

Book

TITLE

Charge and Field Effects in Biosystems—3

ISBN

978-1-4615-9839-8
978-1-4615-9837-4

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4615-9837-4_24

DOI

http://dx.doi.org/10.1007/978-1-4615-9837-4_24

DIMENSIONS

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