Oxidative Base Damage to DNA View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1999

AUTHORS

J. Cadet , M. Bardet , M. Berger , T. Berthod , T. Delatour , C. D’Ham , T. Douki , D. Gasparutto , A. Grand , A. Guy , F. Jolibois , D. Molko , M. Polverelli , J.-L. Ravanat , A. Romieu , N. Signorini , S. Sauvaigo

ABSTRACT

Emphasis was placed in this short survey on recent chemical and biochemical aspects of oxidative base damage to DNA. Significant progress has been accomplished in a better understanding of the oxidation reactions mediated by OH radical, singlet oxygen and one-electron oxidation of the guanine moiety of DNA and model compounds. As a second major topic, relevant information was recently gained on the origin of major artifacts in the measurement (gas chromatography-mass spectrometry, high performane liquid chromatography-[32P]-postlabeling and immunological assays) of oxidative base lesions in cellular DNA. Prepurification of the targeted base damage prior to derivatization and enzymatic phosphorylation is a requisite to prevent artifactual oxidation to occur in the two former assays. Finally insight is provided on the specificity of repair by DNA-glycosylases using site specific modified oligonucleotides More... »

PAGES

47-58

References to SciGraph publications

  • 1993-04. Instability and decay of the primary structure of DNA in NATURE
  • 1996-06. Cancer risk and oxidative DNA damage in man in JOURNAL OF MOLECULAR MEDICINE
  • 2006-02-19. DNA damage profiles induced by oxidizing agents in REVIEWS OF PHYSIOLOGY, BIOCHEMISTRY AND PHARMACOLOGY, VOLUME 94
  • Book

    TITLE

    Advances in DNA Damage and Repair

    ISBN

    978-1-4613-7207-3
    978-1-4615-4865-2

    Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/978-1-4615-4865-2_5

    DOI

    http://dx.doi.org/10.1007/978-1-4615-4865-2_5

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1028200249


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