Genome Analysis in Brassica Using RFLPs View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1991

AUTHORS

Thomas C. Osborn , Keming M. Song , Wayne C. Kennard , Mary K. Slocum , Scott Figdore , Jon Suzuki , Paul H. Williams

ABSTRACT

The analysis of plant genomes has been advanced greatly by using genetic information derived from restriction fragment length polymorphism (RFLP) of nuclear DNAs. RFLPs can be detected by digesting plant DNAs with restriction endonucleases and hybridizing Southern blots of the DNAs with labeled clones of low copy nuclear sequences. Homologous restriction fragments which vary in length can be distinguished as different molecular weight bands, and in segregating progenies, these RFLPs behave as simple Mendelian markers. RFLPs have been used to construct detailed linkage maps in several plant species, providing information on the organization and relationships of plant genomes. They also have been used to locate and study the effects of genes controlling traits of interest. They are particularly useful for analyzing quantitative traits which are under complex genetic control and influenced by the environment. These and other applications of RFLPs have great potential for assisting plant breeders in the improvement of crop species (Beckmann and Soller, 1986; Tanksley et al., 1989). More... »

PAGES

269-276

Book

TITLE

Plant Molecular Biology 2

ISBN

978-1-4613-6454-2
978-1-4615-3304-7

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4615-3304-7_28

DOI

http://dx.doi.org/10.1007/978-1-4615-3304-7_28

DIMENSIONS

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