Soybean Genome Analysis: DNA Polymorphisms are Identified by Oligonucleotide Primers of Arbitrary Sequence View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1991

AUTHORS

Scott V. Tingey , J. Antoni Rafalski , John G. K. Williams , Scott Sebastian

ABSTRACT

We have recently constructed a complete linkage map of Glycine max using restriction fragment length polymorphism technology. The map contains more than 550 markers which describe 23 linkage groups and 2700 cM of DNA. Given the level of polymorphism between soybean cultivars, for a single genetic map to have general utility in a breeding program, the map must contain over 1300 markers. To reach this goal, we have developed new technology for genetic mapping that promises to automate genotype determination. The new assay is called a RAPD assay after Random Amplified Polymorphic DNA. It is based on the observation that single oligonucleotide primers of arbitrary sequence can be used to amplify discrete loci in a complex genome. More... »

PAGES

263-268

Book

TITLE

Plant Molecular Biology 2

ISBN

978-1-4613-6454-2
978-1-4615-3304-7

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4615-3304-7_27

DOI

http://dx.doi.org/10.1007/978-1-4615-3304-7_27

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1016877271


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