Single Cell Analysis of Factors Increasing the Survival of Resealed Erythrocytes in the Circulation of Mice View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1992

AUTHORS

Robert A. Schlegel , Katherine Lumley-Sapanski , Patrick Williamson

ABSTRACT

Erythrocytes labeled with fluorescent membrane markers were reinfused into mice. Blood samples removed periodically were analyzed by flow cytometry for survival of labeled cells. Normal erythrocytes labeled with the markers persisted in the circulation for 45 days. A fraction of lysed and resealed erythrocytes labeled with marker were rapidly cleared from the circulation; the remainder were removed at approximately the same rate as normal cells in the same animal labeled with marker of another color. Lysis in a minimal volume of buffer and addition of ATP during lysis both increased the fraction of cells surviving initial clearance. More... »

PAGES

133-138

References to SciGraph publications

Book

TITLE

The Use of Resealed Erythrocytes as Carriers and Bioreactors

ISBN

978-1-4613-6321-7
978-1-4615-3030-5

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4615-3030-5_16

DOI

http://dx.doi.org/10.1007/978-1-4615-3030-5_16

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1000685925


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