Ontology type: schema:Chapter Open Access: True
1994
AUTHORSFumihiro Taguchi , Toshio Ikeda , Keiichi Saeki , Hideyuki Kubo , Tateki Kikuchi
ABSTRACTWe have tested the fusogenic properties of cleaved and uncleaved spike (S) protein of mutine coronavirus (MCV) JHMV variant cl-2 by expressing the S protein by recombinant vaccinia viruses (RVVs). The amino acid sequence of the putative cleavage site of cl-2 S protein, Arg-Arg-Ala-Arg-Arg, was replaced by Arg-Thr-Ala-Leu-Glu by in vitro mutagenesis of cl-2 S gene. The RVVs having cl-2 S gene [RVV t(+)] or mutated cl-2 S gene [RVV t(-)] were tested for their ability to induce fusion as well as cleavability in DBT cells. After inoculation with RVV t(+) onto DBT cells, the fusion formation was first observed at 8 h postinoculation (p.i.) and spread throughout the whole culture by 24 h. In cells infected with RVV t(-), fusion appeared by 2 h and most of cells were fused by 30 h p.i. The S protein and its cleavage products were detected in DBT cells expressing wild type S protein. However, no cleavage products of the S protein were detected in RVV t(-) infected cells producing mutated S protein, even though fusion was clearly visible. These results suggest that the cleavage event of JHMV-S protein of MCV is not a prerequisite for fusion formation, but that it enhances fusion. More... »
PAGES171-175
Coronaviruses
ISBN
978-1-4613-6305-7
978-1-4615-2996-5
http://scigraph.springernature.com/pub.10.1007/978-1-4615-2996-5_27
DOIhttp://dx.doi.org/10.1007/978-1-4615-2996-5_27
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PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/8209726
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