Ontology type: schema:Chapter Open Access: True
1994
AUTHORSFumihiro Taguchi , Toshio Ikeda , Keiichi Saeki , Hideyuki Kubo , Tateki Kikuchi
ABSTRACTWe have tested the fusogenic properties of cleaved and uncleaved spike (S) protein of mutine coronavirus (MCV) JHMV variant cl-2 by expressing the S protein by recombinant vaccinia viruses (RVVs). The amino acid sequence of the putative cleavage site of cl-2 S protein, Arg-Arg-Ala-Arg-Arg, was replaced by Arg-Thr-Ala-Leu-Glu by in vitro mutagenesis of cl-2 S gene. The RVVs having cl-2 S gene [RVV t(+)] or mutated cl-2 S gene [RVV t(-)] were tested for their ability to induce fusion as well as cleavability in DBT cells. After inoculation with RVV t(+) onto DBT cells, the fusion formation was first observed at 8 h postinoculation (p.i.) and spread throughout the whole culture by 24 h. In cells infected with RVV t(-), fusion appeared by 2 h and most of cells were fused by 30 h p.i. The S protein and its cleavage products were detected in DBT cells expressing wild type S protein. However, no cleavage products of the S protein were detected in RVV t(-) infected cells producing mutated S protein, even though fusion was clearly visible. These results suggest that the cleavage event of JHMV-S protein of MCV is not a prerequisite for fusion formation, but that it enhances fusion. More... »
PAGES171-175
Coronaviruses
ISBN
978-1-4613-6305-7
978-1-4615-2996-5
http://scigraph.springernature.com/pub.10.1007/978-1-4615-2996-5_27
DOIhttp://dx.doi.org/10.1007/978-1-4615-2996-5_27
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1001030813
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/8209726
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Medical and Health Sciences",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Amino Acid Sequence",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Animals",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Astrocytoma",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Base Sequence",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cell Fusion",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cytopathogenic Effect, Viral",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Endopeptidases",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Membrane Glycoproteins",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Mice",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Molecular Sequence Data",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Murine hepatitis virus",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Mutagenesis, Site-Directed",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Protein Processing, Post-Translational",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Spike Glycoprotein, Coronavirus",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Tumor Cells, Cultured",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Viral Envelope Proteins",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "National Institute of Neuroscience, NCNP, 4-1-1 Ogawahigashi, Kodaira, Tokyo 187, Japan",
"id": "http://www.grid.ac/institutes/grid.419280.6",
"name": [
"National Institute of Neuroscience, NCNP, 4-1-1 Ogawahigashi, Kodaira, Tokyo 187, Japan"
],
"type": "Organization"
},
"familyName": "Taguchi",
"givenName": "Fumihiro",
"id": "sg:person.0607142235.90",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0607142235.90"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "National Institute of Neuroscience, NCNP, 4-1-1 Ogawahigashi, Kodaira, Tokyo 187, Japan",
"id": "http://www.grid.ac/institutes/grid.419280.6",
"name": [
"National Institute of Neuroscience, NCNP, 4-1-1 Ogawahigashi, Kodaira, Tokyo 187, Japan"
],
"type": "Organization"
},
"familyName": "Ikeda",
"givenName": "Toshio",
"id": "sg:person.0737512614.15",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0737512614.15"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "National Institute of Neuroscience, NCNP, 4-1-1 Ogawahigashi, Kodaira, Tokyo 187, Japan",
"id": "http://www.grid.ac/institutes/grid.419280.6",
"name": [
"National Institute of Neuroscience, NCNP, 4-1-1 Ogawahigashi, Kodaira, Tokyo 187, Japan"
],
"type": "Organization"
},
"familyName": "Saeki",
"givenName": "Keiichi",
"id": "sg:person.01027170254.41",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01027170254.41"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "National Institute of Neuroscience, NCNP, 4-1-1 Ogawahigashi, Kodaira, Tokyo 187, Japan",
"id": "http://www.grid.ac/institutes/grid.419280.6",
"name": [
"National Institute of Neuroscience, NCNP, 4-1-1 Ogawahigashi, Kodaira, Tokyo 187, Japan"
],
"type": "Organization"
},
"familyName": "Kubo",
"givenName": "Hideyuki",
"id": "sg:person.01075303454.54",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01075303454.54"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "National Institute of Neuroscience, NCNP, 4-1-1 Ogawahigashi, Kodaira, Tokyo 187, Japan",
"id": "http://www.grid.ac/institutes/grid.419280.6",
"name": [
"National Institute of Neuroscience, NCNP, 4-1-1 Ogawahigashi, Kodaira, Tokyo 187, Japan"
],
"type": "Organization"
},
"familyName": "Kikuchi",
"givenName": "Tateki",
"id": "sg:person.011064726622.01",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011064726622.01"
],
"type": "Person"
}
],
"datePublished": "1994",
"datePublishedReg": "1994-01-01",
"description": "We have tested the fusogenic properties of cleaved and uncleaved spike (S) protein of mutine coronavirus (MCV) JHMV variant cl-2 by expressing the S protein by recombinant vaccinia viruses (RVVs). The amino acid sequence of the putative cleavage site of cl-2 S protein, Arg-Arg-Ala-Arg-Arg, was replaced by Arg-Thr-Ala-Leu-Glu by in vitro mutagenesis of cl-2 S gene. The RVVs having cl-2 S gene [RVV t(+)] or mutated cl-2 S gene [RVV t(-)] were tested for their ability to induce fusion as well as cleavability in DBT cells. After inoculation with RVV t(+) onto DBT cells, the fusion formation was first observed at 8 h postinoculation (p.i.) and spread throughout the whole culture by 24 h. In cells infected with RVV t(-), fusion appeared by 2 h and most of cells were fused by 30 h p.i. The S protein and its cleavage products were detected in DBT cells expressing wild type S protein. However, no cleavage products of the S protein were detected in RVV t(-) infected cells producing mutated S protein, even though fusion was clearly visible. These results suggest that the cleavage event of JHMV-S protein of MCV is not a prerequisite for fusion formation, but that it enhances fusion.",
"editor": [
{
"familyName": "Laude",
"givenName": "Hubert",
"type": "Person"
},
{
"familyName": "Vautherot",
"givenName": "Jean-Fran\u00e7ois",
"type": "Person"
}
],
"genre": "chapter",
"id": "sg:pub.10.1007/978-1-4615-2996-5_27",
"inLanguage": "en",
"isAccessibleForFree": true,
"isPartOf": {
"isbn": [
"978-1-4613-6305-7",
"978-1-4615-2996-5"
],
"name": "Coronaviruses",
"type": "Book"
},
"keywords": [
"S protein",
"S gene",
"DBT cells",
"recombinant vaccinia virus",
"fusogenic properties",
"wild-type S protein",
"putative cleavage site",
"murine coronavirus JHMV",
"cleavage products",
"fusion formation",
"spike protein",
"cleavage events",
"cleavage site",
"protein",
"genes",
"Arg-Arg",
"Ala-Arg",
"h postinoculation",
"cells",
"vaccinia virus",
"Arg-Thr",
"Ala-Leu",
"fusion",
"mutagenesis",
"whole culture",
"JHMV variants",
"sequence",
"Arg",
"Glu",
"inoculation",
"cleavability",
"variants",
"formation",
"sites",
"virus",
"postinoculation",
"culture",
"products",
"prerequisite",
"ability",
"JHMV",
"events",
"results",
"properties",
"MCV"
],
"name": "Fusogenic Properties of Uncleaved Spike Protein of Murine Coronavirus JHMV",
"pagination": "171-175",
"productId": [
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1001030813"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/978-1-4615-2996-5_27"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"8209726"
]
}
],
"publisher": {
"name": "Springer Nature",
"type": "Organisation"
},
"sameAs": [
"https://doi.org/10.1007/978-1-4615-2996-5_27",
"https://app.dimensions.ai/details/publication/pub.1001030813"
],
"sdDataset": "chapters",
"sdDatePublished": "2022-05-20T07:46",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-springernature-scigraph/baseset/20220519/entities/gbq_results/chapter/chapter_35.jsonl",
"type": "Chapter",
"url": "https://doi.org/10.1007/978-1-4615-2996-5_27"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/978-1-4615-2996-5_27'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/978-1-4615-2996-5_27'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/978-1-4615-2996-5_27'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/978-1-4615-2996-5_27'
This table displays all metadata directly associated to this object as RDF triples.
202 TRIPLES
23 PREDICATES
87 URIs
81 LITERALS
24 BLANK NODES