Genotyping of Human Papillomavirus (HPV) by Single-Strand Conformational Polymorphism (SSCP) View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1994

AUTHORS

Ingeborg Zehbe , Eva Rylander , Jan Sällström , Erik Wilander

ABSTRACT

Nowadays, the polymerase chain reaction (PCR) is the most sensitive and widely used method for the detection of human papillomavirus (HPV) using ethidium bromide gel electrophoresis together with hybridization assays for typing. In this study, we demonstrate single-strand conformational. polymorphism (SSCP) as simultaneous detection and typing method for PCR-amplified products. SSCP as first described by Orita et al.l,2 employs denaturation of double strand DNA into single strand DNA and separation of the products by polyacrylamide gel electrophoresis (PAGE). This method makes possible the detection of HPV-DNA conformational. changes resulting in indivual. band patterns of the most common HPV-types. More... »

PAGES

55-58

Book

TITLE

Immunology of Human Papillomaviruses

ISBN

978-1-4613-6041-4
978-1-4615-2449-6

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4615-2449-6_10

DOI

http://dx.doi.org/10.1007/978-1-4615-2449-6_10

DIMENSIONS

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