Early B Cell Differentiation from Hematopoietic Stem Cells in the Presence of Stromal Cells and Interleukin-7 (IL-7) View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1990

AUTHORS

T. Suda , A. Ohara , J. Suda , S. Okada , N. Tokuyama , Y. Miura , T. Sudo , S. I. Nishikawa , H. Nakauchi

ABSTRACT

The early stage of the pathway by which lymphocytes differentiate from hematopoietic stem cells was studied at a clonal level, using a stromal cell line (ST-2). IL-3-induced blast colonies, shown to be capable of differentiation into a variety of hematopoietic cells, were used as a source of enriched hematopoietic progenitor cells. These cells were Thy-1+ and B220−. In 7 of 211 wells receiving each blast colony, lymphoid cell and myeloid cell growth was observed on ST-2 layer. The cells were proved to be B lymphocytes by phenotype and immunoglobulin gene rearrangement analysis and by demonstration of surface expression of IgM. The clonal origin of B lymphoid and myeloid lineage cells was confirmed by the generation of both B lymphoid and myeloid cells in the same well following a fluorescence-activate cell sorter (FACS) clone sorting of IL-3-induced blast cells. These results provide evidence that cells of B lymphoid and myeloid lineage can originate from single primitive hematopoieitc stem cells.Northern blot analysis revealed that this stromal cell line produced IL-7 by the stimulation of the contract with pre-B cells. We showed that IL-7 acted on B220+, IgM− cells and supported the colony formation. These colony cells were exclusively B220+, and some of cells were IgM+ and Thy1+. Thus, it may be concluded that differentiation from B220− cells into B220+ cells were supported by stromal cells or stroma derived factors, but not by IL-7. IL-7 can support the second step of differentiation of B220+, IgM- cells into B220+, IgM+ cells. More... »

PAGES

45-52

Book

TITLE

Experimental Hematology Today—1989

ISBN

978-1-4613-9005-3
978-1-4613-9003-9

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4613-9003-9_8

DOI

http://dx.doi.org/10.1007/978-1-4613-9003-9_8

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1018555521


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