Gata Transcription Factors Negatively Regulate Erythropoietin Gene Expression View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1996

AUTHORS

Shigehiko Imagawa , Masayuki Yamamoto , Yasusada Miura

ABSTRACT

We examined regulation of the human erythropoietin (Epo) gene through the GATA sequence in the Epo promoter, and demonstrated that Hep3B and HepG2 cells express human GATA-2 (hGATA-2) mRNA and protein. Nuclear extracts of QT6 cells transfected with hGATA-1, 2 or 3 transcription factors revealed specific binding to the GATA element in the human Epo gene promoter by gel mobility shift assay. Transient transfection of Hep3B cells with hGATA-1, 2 or 3 demonstrated that each of these transcription factors significantly decreased the level of expression of Epo mRNA as assessed by a competitive polymerase chain reaction (PCR). Furthermore, transient transfection of Hep3B cells with hGATA-1, 2 and 3 and an Epo-reporter gene construct showed significant inhibition of the Epo promoter. We conclude that the hGATA-1,2 and 3 transcription factors specifically bind to the GATA element in the human Epo gene promoter and negatively regulate Epo gene expression. More... »

PAGES

501-513

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4613-0391-6_61

DOI

http://dx.doi.org/10.1007/978-1-4613-0391-6_61

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1014173662


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