Purification of L-Kynurenine 3-Monooxygenase from Pig Liver Mitochondrial Outer Membrane View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1996

AUTHORS

T. Uemura , K. Hirai

ABSTRACT

L-Kynurenine 3-monooxygenase is an FAD-dependent mixed function oxidase located in the mitochondrial outer membrane (Okamoto and Hayaishi, 1969). The purification of the mammalian enzyme has been done only from rat liver mitochondria (Horn et al., 1971; Nishimoto et al., 1977; 1979) and never from other mammalian livers. In this paper we describe the method for the purification of this enzyme from pig liver mitochondrial outer membrane. It consists of (1) the extraction of the enzyme from the Chaps*-insoluble outer membrane material by the nonionic detergent, β-Octylglucoside, (2) ammonium sulfate fractionation, (3) DEAE-Sepharose CL-6B chromatography, and (4) affinity chromatography (mercurial-Sepharose). More... »

PAGES

527-530

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4613-0381-7_82

DOI

http://dx.doi.org/10.1007/978-1-4613-0381-7_82

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1025681695

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/8906316


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