Ontology type: schema:Chapter
1996
AUTHORS ABSTRACTL-Kynurenine 3-monooxygenase is an FAD-dependent mixed function oxidase located in the mitochondrial outer membrane (Okamoto and Hayaishi, 1969). The purification of the mammalian enzyme has been done only from rat liver mitochondria (Horn et al., 1971; Nishimoto et al., 1977; 1979) and never from other mammalian livers. In this paper we describe the method for the purification of this enzyme from pig liver mitochondrial outer membrane. It consists of (1) the extraction of the enzyme from the Chaps*-insoluble outer membrane material by the nonionic detergent, β-Octylglucoside, (2) ammonium sulfate fractionation, (3) DEAE-Sepharose CL-6B chromatography, and (4) affinity chromatography (mercurial-Sepharose). More... »
PAGES527-530
Recent Advances in Tryptophan Research
ISBN
978-1-4613-8026-9
978-1-4613-0381-7
http://scigraph.springernature.com/pub.10.1007/978-1-4613-0381-7_82
DOIhttp://dx.doi.org/10.1007/978-1-4613-0381-7_82
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PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/8906316
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