Quantitative PCR Technology View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1998

AUTHORS

Lincoln McBride , Ken Livak , Mike Lucero , Federico Goodsaid , Dane Carlson , Junko Stevens , Traci Allen , Paul Wyatt , Daniel Thiel , Peter Honebein , John Shigeura , Tim Woudenberg , Eugene Young , Raymond Lefebvre , Susan Flood , Bruce Goldman , Jeff Lucas , Kevin Bodner , Robert Grossman , Bashar Mullah , Charles Connel , Linda Lee , Mark Oldham

ABSTRACT

Higuchi et al. (1992, 1993) pioneered the analysis of PCR kinetics by constructing a system that detects PCR products as they accumulate. This “real-time” system includes the intercalator ethidium bromide in each amplification reaction, an adapted thermal cycler to irradiate the samples with ultraviolet light, and detection of the resulting fluorescence with a computer-controlled cooled CCD camera. Amplification produces increasing amounts of double-stranded DNA, which binds ethidium bromide, resulting in an increase in fluorescence. By plotting the increase in fluorescence versus cycle number, the system produces amplification plots that provide a more complete picture of the PCR process than assaying product accumulation after a fixed number of cycles. More... »

PAGES

97-110

References to SciGraph publications

Book

TITLE

Gene Quantification

ISBN

978-1-4612-8682-0
978-1-4612-4164-5

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4612-4164-5_6

DOI

http://dx.doi.org/10.1007/978-1-4612-4164-5_6

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1005566779


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