CK2α — protein phosphatase 2A molecular complex: Possible interaction with the MAP kinase pathway View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1999

AUTHORS

Franck Lebrin , Laurence Bianchini , Thierry Rabilloud , Edmond M. Chambaz , Yves Goldberg

ABSTRACT

Despite its wide range of known substrates, the signaling function of protein kinase CK2 is still enigmatic. Mounting evidence suggests that CK2α, the catalytic subunit of holoenzymic CK2, may exist free of its usual regulatory partner CK2β, raising the possibility that ‘free’ CK2α has regulation and function distinct from those of the holoenzyme. We previously reported that CK2α could bind to the core dimer of protein phosphatase 2 A, and indirectly cause down-regulation of the PP2A substrate MEK1, possibly via activation of PP2A and/or targeting of PP2A to some element of the Ras/Raf/MEK pathway. Here, these results are confirmed and extended. By using transfection experiments and immune kinase assays, we show that endogenous PP2Ac and CK2β are the only major substrates associating with epitope-tagged CK2α, and that expression of activated Raf results in disruption of the CK2α-PP2A association. Such disruption might be a necessary step for maximal activation of the MAP kinase pathway by Raf. In keeping with this idea, overexpression of CK2α dose-dependently inhibits the mitogen-induced activation of cotransfected, epitope-tagged MAP kinase. We suggest that the CK2β free form of CK2α is both a target and a regulator of Raf/MAPK signaling. (Mol Cell Biochem 191: 207–212, 1999) More... »

PAGES

207-212

Book

TITLE

A Molecular and Cellular View of Protein Kinase CK2

ISBN

978-1-4613-4648-7
978-1-4419-8624-5

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4419-8624-5_25

DOI

http://dx.doi.org/10.1007/978-1-4419-8624-5_25

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1024917727


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "name": [
            "INSERM U244 and CEA/Grenoble, DBMS-BRCE, Grenoble, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lebrin", 
        "givenName": "Franck", 
        "id": "sg:person.0617422204.34", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0617422204.34"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "INSERM U244 and CEA/Grenoble, DBMS-BRCE, Grenoble, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bianchini", 
        "givenName": "Laurence", 
        "id": "sg:person.0676353402.29", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0676353402.29"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "CEA Grenoble", 
          "id": "https://www.grid.ac/institutes/grid.457348.9", 
          "name": [
            "CEA/Grenoble, DBMS-BECP, Grenoble, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Rabilloud", 
        "givenName": "Thierry", 
        "id": "sg:person.0605652034.21", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0605652034.21"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "INSERM U244 and CEA/Grenoble, DBMS-BRCE, Grenoble, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Chambaz", 
        "givenName": "Edmond M.", 
        "id": "sg:person.01012217512.31", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01012217512.31"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "INSERM U244 and CEA/Grenoble, DBMS-BRCE, Grenoble, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Goldberg", 
        "givenName": "Yves", 
        "id": "sg:person.01155070114.86", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01155070114.86"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1006/abbi.1993.1037", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017584153"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1091/mbc.3.1.63", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024729415"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(95)90405-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030663768"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1432-1033.1992.tb16636.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034754320"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(93)90533-v", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043750692"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.276.5314.952", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062556652"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.7846532", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062649899"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077330402", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082485761", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1096/fasebj.9.5.7896000", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1082555158"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082660162", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1999", 
    "datePublishedReg": "1999-01-01", 
    "description": "Despite its wide range of known substrates, the signaling function of protein kinase CK2 is still enigmatic. Mounting evidence suggests that CK2\u03b1, the catalytic subunit of holoenzymic CK2, may exist free of its usual regulatory partner CK2\u03b2, raising the possibility that \u2018free\u2019 CK2\u03b1 has regulation and function distinct from those of the holoenzyme. We previously reported that CK2\u03b1 could bind to the core dimer of protein phosphatase 2 A, and indirectly cause down-regulation of the PP2A substrate MEK1, possibly via activation of PP2A and/or targeting of PP2A to some element of the Ras/Raf/MEK pathway. Here, these results are confirmed and extended. By using transfection experiments and immune kinase assays, we show that endogenous PP2Ac and CK2\u03b2 are the only major substrates associating with epitope-tagged CK2\u03b1, and that expression of activated Raf results in disruption of the CK2\u03b1-PP2A association. Such disruption might be a necessary step for maximal activation of the MAP kinase pathway by Raf. In keeping with this idea, overexpression of CK2\u03b1 dose-dependently inhibits the mitogen-induced activation of cotransfected, epitope-tagged MAP kinase. We suggest that the CK2\u03b2 free form of CK2\u03b1 is both a target and a regulator of Raf/MAPK signaling. (Mol Cell Biochem 191: 207\u2013212, 1999)", 
    "editor": [
      {
        "familyName": "Ahmed", 
        "givenName": "Khalil", 
        "type": "Person"
      }, 
      {
        "familyName": "Issinger", 
        "givenName": "O. G.", 
        "type": "Person"
      }, 
      {
        "familyName": "Chambaz", 
        "givenName": "E.", 
        "type": "Person"
      }
    ], 
    "genre": "chapter", 
    "id": "sg:pub.10.1007/978-1-4419-8624-5_25", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": {
      "isbn": [
        "978-1-4613-4648-7", 
        "978-1-4419-8624-5"
      ], 
      "name": "A Molecular and Cellular View of Protein Kinase CK2", 
      "type": "Book"
    }, 
    "name": "CK2\u03b1 \u2014 protein phosphatase 2A molecular complex: Possible interaction with the MAP kinase pathway", 
    "pagination": "207-212", 
    "productId": [
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/978-1-4419-8624-5_25"
        ]
      }, 
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "76b57d7f79e1b7a34a155ca31840a795a04969c9651750313a1a22227b93293c"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1024917727"
        ]
      }
    ], 
    "publisher": {
      "location": "Boston, MA", 
      "name": "Springer US", 
      "type": "Organisation"
    }, 
    "sameAs": [
      "https://doi.org/10.1007/978-1-4419-8624-5_25", 
      "https://app.dimensions.ai/details/publication/pub.1024917727"
    ], 
    "sdDataset": "chapters", 
    "sdDatePublished": "2019-04-15T15:21", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8672_00000258.jsonl", 
    "type": "Chapter", 
    "url": "http://link.springer.com/10.1007/978-1-4419-8624-5_25"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/978-1-4419-8624-5_25'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/978-1-4419-8624-5_25'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/978-1-4419-8624-5_25'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/978-1-4419-8624-5_25'


 

This table displays all metadata directly associated to this object as RDF triples.

141 TRIPLES      23 PREDICATES      38 URIs      20 LITERALS      8 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/978-1-4419-8624-5_25 schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author N28635380717d42b4b6da7324619f592e
4 schema:citation https://app.dimensions.ai/details/publication/pub.1077330402
5 https://app.dimensions.ai/details/publication/pub.1082485761
6 https://app.dimensions.ai/details/publication/pub.1082660162
7 https://doi.org/10.1006/abbi.1993.1037
8 https://doi.org/10.1016/0092-8674(93)90533-v
9 https://doi.org/10.1016/0092-8674(95)90405-0
10 https://doi.org/10.1091/mbc.3.1.63
11 https://doi.org/10.1096/fasebj.9.5.7896000
12 https://doi.org/10.1111/j.1432-1033.1992.tb16636.x
13 https://doi.org/10.1126/science.276.5314.952
14 https://doi.org/10.1126/science.7846532
15 schema:datePublished 1999
16 schema:datePublishedReg 1999-01-01
17 schema:description Despite its wide range of known substrates, the signaling function of protein kinase CK2 is still enigmatic. Mounting evidence suggests that CK2α, the catalytic subunit of holoenzymic CK2, may exist free of its usual regulatory partner CK2β, raising the possibility that ‘free’ CK2α has regulation and function distinct from those of the holoenzyme. We previously reported that CK2α could bind to the core dimer of protein phosphatase 2 A, and indirectly cause down-regulation of the PP2A substrate MEK1, possibly via activation of PP2A and/or targeting of PP2A to some element of the Ras/Raf/MEK pathway. Here, these results are confirmed and extended. By using transfection experiments and immune kinase assays, we show that endogenous PP2Ac and CK2β are the only major substrates associating with epitope-tagged CK2α, and that expression of activated Raf results in disruption of the CK2α-PP2A association. Such disruption might be a necessary step for maximal activation of the MAP kinase pathway by Raf. In keeping with this idea, overexpression of CK2α dose-dependently inhibits the mitogen-induced activation of cotransfected, epitope-tagged MAP kinase. We suggest that the CK2β free form of CK2α is both a target and a regulator of Raf/MAPK signaling. (Mol Cell Biochem 191: 207–212, 1999)
18 schema:editor Nc96b263849794a159b4e8b6e77d9d528
19 schema:genre chapter
20 schema:inLanguage en
21 schema:isAccessibleForFree false
22 schema:isPartOf Nf08456dc39c648ef8aa571599055b1f0
23 schema:name CK2α — protein phosphatase 2A molecular complex: Possible interaction with the MAP kinase pathway
24 schema:pagination 207-212
25 schema:productId N0b9284d823454d1b957de8784fe7e722
26 N73794877d49f449187040eedc11d27cf
27 Naa6ac7c9afc44eb4b866caa5ca91e114
28 schema:publisher N4a6ab4a83e5b409ea6a24628b9361d51
29 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024917727
30 https://doi.org/10.1007/978-1-4419-8624-5_25
31 schema:sdDatePublished 2019-04-15T15:21
32 schema:sdLicense https://scigraph.springernature.com/explorer/license/
33 schema:sdPublisher N9a7730a2a7e0437ea3e2ea394a570d42
34 schema:url http://link.springer.com/10.1007/978-1-4419-8624-5_25
35 sgo:license sg:explorer/license/
36 sgo:sdDataset chapters
37 rdf:type schema:Chapter
38 N02fea9836bce4728914c283b360d69fa rdf:first Nee46ab13ca7849feb34dff52445c10d0
39 rdf:rest rdf:nil
40 N0b9284d823454d1b957de8784fe7e722 schema:name doi
41 schema:value 10.1007/978-1-4419-8624-5_25
42 rdf:type schema:PropertyValue
43 N28635380717d42b4b6da7324619f592e rdf:first sg:person.0617422204.34
44 rdf:rest Nc9f58f1b655e4b75ae22b50a05509eeb
45 N490626a3394247459a4dafb196a7a46a schema:familyName Issinger
46 schema:givenName O. G.
47 rdf:type schema:Person
48 N4a6ab4a83e5b409ea6a24628b9361d51 schema:location Boston, MA
49 schema:name Springer US
50 rdf:type schema:Organisation
51 N73794877d49f449187040eedc11d27cf schema:name dimensions_id
52 schema:value pub.1024917727
53 rdf:type schema:PropertyValue
54 N89050f98519c494eba5325f9599384dc schema:familyName Ahmed
55 schema:givenName Khalil
56 rdf:type schema:Person
57 N9704948ae8404127a238a3da592a446d schema:name INSERM U244 and CEA/Grenoble, DBMS-BRCE, Grenoble, France
58 rdf:type schema:Organization
59 N9963f8c0f8b64e01bfa59946be5b2d70 rdf:first sg:person.0605652034.21
60 rdf:rest Nfb4ded18d31042428f7b7547e6040892
61 N9a7730a2a7e0437ea3e2ea394a570d42 schema:name Springer Nature - SN SciGraph project
62 rdf:type schema:Organization
63 N9c17fa72c8974d83abed326ffb0c3d58 schema:name INSERM U244 and CEA/Grenoble, DBMS-BRCE, Grenoble, France
64 rdf:type schema:Organization
65 Naa6ac7c9afc44eb4b866caa5ca91e114 schema:name readcube_id
66 schema:value 76b57d7f79e1b7a34a155ca31840a795a04969c9651750313a1a22227b93293c
67 rdf:type schema:PropertyValue
68 Nc96b263849794a159b4e8b6e77d9d528 rdf:first N89050f98519c494eba5325f9599384dc
69 rdf:rest Ncf08c4009cfc48c3a3912a2808b47b88
70 Nc9f58f1b655e4b75ae22b50a05509eeb rdf:first sg:person.0676353402.29
71 rdf:rest N9963f8c0f8b64e01bfa59946be5b2d70
72 Ncae2c3f6ac4a48369df2ada2d6a5f799 schema:name INSERM U244 and CEA/Grenoble, DBMS-BRCE, Grenoble, France
73 rdf:type schema:Organization
74 Ncf08c4009cfc48c3a3912a2808b47b88 rdf:first N490626a3394247459a4dafb196a7a46a
75 rdf:rest N02fea9836bce4728914c283b360d69fa
76 Nee46ab13ca7849feb34dff52445c10d0 schema:familyName Chambaz
77 schema:givenName E.
78 rdf:type schema:Person
79 Nf08456dc39c648ef8aa571599055b1f0 schema:isbn 978-1-4419-8624-5
80 978-1-4613-4648-7
81 schema:name A Molecular and Cellular View of Protein Kinase CK2
82 rdf:type schema:Book
83 Nf6d298cc6a994f59978dab4aa24580ec schema:name INSERM U244 and CEA/Grenoble, DBMS-BRCE, Grenoble, France
84 rdf:type schema:Organization
85 Nfb4ded18d31042428f7b7547e6040892 rdf:first sg:person.01012217512.31
86 rdf:rest Nfcc44f5a8d654ba19abe1241c0adaba7
87 Nfcc44f5a8d654ba19abe1241c0adaba7 rdf:first sg:person.01155070114.86
88 rdf:rest rdf:nil
89 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
90 schema:name Biological Sciences
91 rdf:type schema:DefinedTerm
92 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
93 schema:name Biochemistry and Cell Biology
94 rdf:type schema:DefinedTerm
95 sg:person.01012217512.31 schema:affiliation Ncae2c3f6ac4a48369df2ada2d6a5f799
96 schema:familyName Chambaz
97 schema:givenName Edmond M.
98 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01012217512.31
99 rdf:type schema:Person
100 sg:person.01155070114.86 schema:affiliation Nf6d298cc6a994f59978dab4aa24580ec
101 schema:familyName Goldberg
102 schema:givenName Yves
103 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01155070114.86
104 rdf:type schema:Person
105 sg:person.0605652034.21 schema:affiliation https://www.grid.ac/institutes/grid.457348.9
106 schema:familyName Rabilloud
107 schema:givenName Thierry
108 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0605652034.21
109 rdf:type schema:Person
110 sg:person.0617422204.34 schema:affiliation N9704948ae8404127a238a3da592a446d
111 schema:familyName Lebrin
112 schema:givenName Franck
113 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0617422204.34
114 rdf:type schema:Person
115 sg:person.0676353402.29 schema:affiliation N9c17fa72c8974d83abed326ffb0c3d58
116 schema:familyName Bianchini
117 schema:givenName Laurence
118 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0676353402.29
119 rdf:type schema:Person
120 https://app.dimensions.ai/details/publication/pub.1077330402 schema:CreativeWork
121 https://app.dimensions.ai/details/publication/pub.1082485761 schema:CreativeWork
122 https://app.dimensions.ai/details/publication/pub.1082660162 schema:CreativeWork
123 https://doi.org/10.1006/abbi.1993.1037 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017584153
124 rdf:type schema:CreativeWork
125 https://doi.org/10.1016/0092-8674(93)90533-v schema:sameAs https://app.dimensions.ai/details/publication/pub.1043750692
126 rdf:type schema:CreativeWork
127 https://doi.org/10.1016/0092-8674(95)90405-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030663768
128 rdf:type schema:CreativeWork
129 https://doi.org/10.1091/mbc.3.1.63 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024729415
130 rdf:type schema:CreativeWork
131 https://doi.org/10.1096/fasebj.9.5.7896000 schema:sameAs https://app.dimensions.ai/details/publication/pub.1082555158
132 rdf:type schema:CreativeWork
133 https://doi.org/10.1111/j.1432-1033.1992.tb16636.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1034754320
134 rdf:type schema:CreativeWork
135 https://doi.org/10.1126/science.276.5314.952 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062556652
136 rdf:type schema:CreativeWork
137 https://doi.org/10.1126/science.7846532 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062649899
138 rdf:type schema:CreativeWork
139 https://www.grid.ac/institutes/grid.457348.9 schema:alternateName CEA Grenoble
140 schema:name CEA/Grenoble, DBMS-BECP, Grenoble, France
141 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...