Quorum Sensing in Root-Associated and Endopytic Diazotrophs View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2008

AUTHORS

M. Schmid , M. Rothballer , B. Hai , A. Fekete , X. Li , M. Englmann , M. Frommberger , P. Schmitt-Kopplin , A. Hartmann

ABSTRACT

Root-associated or endophytic diazotrophs, like Azospirillum, Herbaspirillum or Burkholderia spp., are of key interest in plant-growth promotion and biological nitrogen-fixation research. Recently, new species of the genera Herbaspirillum and Burkholderia were described (Reis et al., 2004; Rothballer et al., 2006). To analyze the diazotrophic or endophytic life style of bacteria, culture dependent methods, like isolation of bacteria from surface sterilized roots in semisolid N-free media, are not sufficient. Most important for the in situ characterization of the endophytic life style are microscopic techniques. For this purpose, species-specific phylogenetic fluorescently labeled oligonucleotide probes (Stoffels et al., 2001) during FISH analysis and the use of GFP-tagged strains in combination with confocal laser scanning microscopy (CLSM) were used. With orthogonal views of confocal image stacks prepared with digital-image analysis tools, the endophytic colonization of plant cells can easily be followed (Rothballer et al., 2003). TEM-based investigations after staining with monospecific polyclonal antibodies were also successfully used (Rothballer et al., unpublished data, 2007). More... »

PAGES

313-315

Book

TITLE

Biological Nitrogen Fixation: Towards Poverty Alleviation through Sustainable Agriculture

ISBN

978-1-4020-8251-1
978-1-4020-8252-8

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4020-8252-8_121

DOI

http://dx.doi.org/10.1007/978-1-4020-8252-8_121

DIMENSIONS

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