Malate and Fumarate Emerge as Key Players in Primary Metabolism: Arabidopsis thaliana Overexpressing C4-NADP-ME Offer a Way to Manipulate the ... View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2008

AUTHORS

Holger Fahnenstich , Mariana Saigo , Carlos Andreo , María F. Drincovich , Ulf-Ingo Flügge , Verónica G. Maurino

ABSTRACT

Maize C4-NADP-malic enzyme was expressed under the control of the CaMV 35S promoter in Arabidopsis thaliana. An increase in the plastidic NADP-ME activity induced no phenotypic differences in long-day growth conditions. Analysis of metabolite levels, however, revealed a disturbed metabolic profile. Dark-induced senescence progressed more rapidly in MEm plants compared to the wild-type. A retardation of senescence in the transgenic lines was gained by exogenous supply of glucose, sucrose and malate, suggesting that the lack of a rapid energy source is likely to be the initial factor leading to the induction of senescence in these plants. A fairly complete picture of primary metabolism assessed by GC-MS and the in vitro metabolic complementation assays allow us to conclude that MEm transgenic plants entered dark induced senescence more rapidly due to an accelerated starvation. Comparison of the data obtained indicated that extremely low levels of malate and fumarate are responsible for the accelerated dark-induced senescence encountered in the MEm plants. Reinforcing previous results, our data indicate that malate and fumarate are key players in the primary metabolism of Arabidopsis thaliana. More... »

PAGES

971-975

Book

TITLE

Photosynthesis. Energy from the Sun

ISBN

978-1-4020-6707-5
978-1-4020-6709-9

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4020-6709-9_212

DOI

http://dx.doi.org/10.1007/978-1-4020-6709-9_212

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1011932733


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