Novel Cell-based Assay for the Detection of Murine type I IfN View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2007

AUTHORS

Mariela Bollati-Fogolín , Werner Müller

ABSTRACT

Type I Interferon (IFN) comprises IFN-alpha and IFN-beta, which are potent biologically active proteins secreted by virus-infected cells. IFN antiviral activity assays were the first type of bioassays developed to measure the relative potency of IFN preparations. Here we report a new virus free, cellbased assay to quantify murine type I IFN. It basically consists of an indicator cell line in which the Cre-recombinase transcription is driven by the IFNinducible Mx1 promoter and, when expressed, deletes a stop cassette upstream of the eGFP coding region, resulting in the expression of eGFP. The percentage of eGFP expressing cells accurately correlates to the amount of type I IFN added to the culture and can easily be monitored. More... »

PAGES

63-65

Book

TITLE

Cell Technology for Cell Products

ISBN

978-1-4020-5475-4

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4020-5476-1_9

DOI

http://dx.doi.org/10.1007/978-1-4020-5476-1_9

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1001153972


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