Ontology type: schema:Chapter
2007
AUTHORSMariela Bollati-Fogolín , Werner Müller
ABSTRACTType I Interferon (IFN) comprises IFN-alpha and IFN-beta, which are potent biologically active proteins secreted by virus-infected cells. IFN antiviral activity assays were the first type of bioassays developed to measure the relative potency of IFN preparations. Here we report a new virus free, cellbased assay to quantify murine type I IFN. It basically consists of an indicator cell line in which the Cre-recombinase transcription is driven by the IFNinducible Mx1 promoter and, when expressed, deletes a stop cassette upstream of the eGFP coding region, resulting in the expression of eGFP. The percentage of eGFP expressing cells accurately correlates to the amount of type I IFN added to the culture and can easily be monitored. More... »
PAGES63-65
Cell Technology for Cell Products
ISBN978-1-4020-5475-4
http://scigraph.springernature.com/pub.10.1007/978-1-4020-5476-1_9
DOIhttp://dx.doi.org/10.1007/978-1-4020-5476-1_9
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