Novel Cell-based Assay for the Detection of Murine type I IfN View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2007

AUTHORS

Mariela Bollati-Fogolín , Werner Müller

ABSTRACT

Type I Interferon (IFN) comprises IFN-alpha and IFN-beta, which are potent biologically active proteins secreted by virus-infected cells. IFN antiviral activity assays were the first type of bioassays developed to measure the relative potency of IFN preparations. Here we report a new virus free, cellbased assay to quantify murine type I IFN. It basically consists of an indicator cell line in which the Cre-recombinase transcription is driven by the IFNinducible Mx1 promoter and, when expressed, deletes a stop cassette upstream of the eGFP coding region, resulting in the expression of eGFP. The percentage of eGFP expressing cells accurately correlates to the amount of type I IFN added to the culture and can easily be monitored. More... »

PAGES

63-65

Book

TITLE

Cell Technology for Cell Products

ISBN

978-1-4020-5475-4

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4020-5476-1_9

DOI

http://dx.doi.org/10.1007/978-1-4020-5476-1_9

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1001153972


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1108", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical Microbiology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "GBF German Research Centre for Biotechnology", 
          "id": "https://www.grid.ac/institutes/grid.418123.d", 
          "name": [
            "Department of Experimental Immunology, German Research Centre for Biotechnology (GBF), Braunschweig, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bollati-Fogol\u00edn", 
        "givenName": "Mariela", 
        "id": "sg:person.01034422411.10", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01034422411.10"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "GBF German Research Centre for Biotechnology", 
          "id": "https://www.grid.ac/institutes/grid.418123.d", 
          "name": [
            "Department of Experimental Immunology, German Research Centre for Biotechnology (GBF), Braunschweig, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "M\u00fcller", 
        "givenName": "Werner", 
        "id": "sg:person.01261504775.94", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01261504775.94"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1002/gene.1030", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018671134"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.7660125", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062648331"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2007", 
    "datePublishedReg": "2007-01-01", 
    "description": "Type I Interferon (IFN) comprises IFN-alpha and IFN-beta, which are potent biologically active proteins secreted by virus-infected cells. IFN antiviral activity assays were the first type of bioassays developed to measure the relative potency of IFN preparations. Here we report a new virus free, cellbased assay to quantify murine type I IFN. It basically consists of an indicator cell line in which the Cre-recombinase transcription is driven by the IFNinducible Mx1 promoter and, when expressed, deletes a stop cassette upstream of the eGFP coding region, resulting in the expression of eGFP. The percentage of eGFP expressing cells accurately correlates to the amount of type I IFN added to the culture and can easily be monitored.", 
    "editor": [
      {
        "familyName": "Smith", 
        "givenName": "Rodney", 
        "type": "Person"
      }
    ], 
    "genre": "chapter", 
    "id": "sg:pub.10.1007/978-1-4020-5476-1_9", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": {
      "isbn": [
        "978-1-4020-5475-4"
      ], 
      "name": "Cell Technology for Cell Products", 
      "type": "Book"
    }, 
    "name": "Novel Cell-based Assay for the Detection of Murine type I IfN", 
    "pagination": "63-65", 
    "productId": [
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/978-1-4020-5476-1_9"
        ]
      }, 
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "057f7540438a16966b58961850fa2da5f2f1c748bbc12946c0064c66d7c97cb4"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1001153972"
        ]
      }
    ], 
    "publisher": {
      "location": "Dordrecht", 
      "name": "Springer Netherlands", 
      "type": "Organisation"
    }, 
    "sameAs": [
      "https://doi.org/10.1007/978-1-4020-5476-1_9", 
      "https://app.dimensions.ai/details/publication/pub.1001153972"
    ], 
    "sdDataset": "chapters", 
    "sdDatePublished": "2019-04-16T00:45", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8700_00000243.jsonl", 
    "type": "Chapter", 
    "url": "http://link.springer.com/10.1007/978-1-4020-5476-1_9"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/978-1-4020-5476-1_9'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/978-1-4020-5476-1_9'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/978-1-4020-5476-1_9'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/978-1-4020-5476-1_9'


 

This table displays all metadata directly associated to this object as RDF triples.

77 TRIPLES      23 PREDICATES      29 URIs      20 LITERALS      8 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/978-1-4020-5476-1_9 schema:about anzsrc-for:11
2 anzsrc-for:1108
3 schema:author Nac240dcd178346d7a5cfce55da065ced
4 schema:citation https://doi.org/10.1002/gene.1030
5 https://doi.org/10.1126/science.7660125
6 schema:datePublished 2007
7 schema:datePublishedReg 2007-01-01
8 schema:description Type I Interferon (IFN) comprises IFN-alpha and IFN-beta, which are potent biologically active proteins secreted by virus-infected cells. IFN antiviral activity assays were the first type of bioassays developed to measure the relative potency of IFN preparations. Here we report a new virus free, cellbased assay to quantify murine type I IFN. It basically consists of an indicator cell line in which the Cre-recombinase transcription is driven by the IFNinducible Mx1 promoter and, when expressed, deletes a stop cassette upstream of the eGFP coding region, resulting in the expression of eGFP. The percentage of eGFP expressing cells accurately correlates to the amount of type I IFN added to the culture and can easily be monitored.
9 schema:editor N7519dd16366d44748f5df293a3c30abe
10 schema:genre chapter
11 schema:inLanguage en
12 schema:isAccessibleForFree false
13 schema:isPartOf Nb16e8845b21f4239a3be98cf3bc35dc2
14 schema:name Novel Cell-based Assay for the Detection of Murine type I IfN
15 schema:pagination 63-65
16 schema:productId N92475ea0d44444ae83593b2451bde2fc
17 Naba01e3a6adb42fa87ba832e05fa127f
18 Nfcfc70a72e5b44a7ac4b3ca9fef0beef
19 schema:publisher Nc1838cbf0b374c7bbf28fe270c13ea44
20 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001153972
21 https://doi.org/10.1007/978-1-4020-5476-1_9
22 schema:sdDatePublished 2019-04-16T00:45
23 schema:sdLicense https://scigraph.springernature.com/explorer/license/
24 schema:sdPublisher Na188b30499554386a436615483c58010
25 schema:url http://link.springer.com/10.1007/978-1-4020-5476-1_9
26 sgo:license sg:explorer/license/
27 sgo:sdDataset chapters
28 rdf:type schema:Chapter
29 N4750f6ab1e0145beb6c21401184b4726 schema:familyName Smith
30 schema:givenName Rodney
31 rdf:type schema:Person
32 N7519dd16366d44748f5df293a3c30abe rdf:first N4750f6ab1e0145beb6c21401184b4726
33 rdf:rest rdf:nil
34 N92475ea0d44444ae83593b2451bde2fc schema:name readcube_id
35 schema:value 057f7540438a16966b58961850fa2da5f2f1c748bbc12946c0064c66d7c97cb4
36 rdf:type schema:PropertyValue
37 Na188b30499554386a436615483c58010 schema:name Springer Nature - SN SciGraph project
38 rdf:type schema:Organization
39 Naba01e3a6adb42fa87ba832e05fa127f schema:name dimensions_id
40 schema:value pub.1001153972
41 rdf:type schema:PropertyValue
42 Nac240dcd178346d7a5cfce55da065ced rdf:first sg:person.01034422411.10
43 rdf:rest Nf5de78a5dad74b56b7607e2d9988c2c3
44 Nb16e8845b21f4239a3be98cf3bc35dc2 schema:isbn 978-1-4020-5475-4
45 schema:name Cell Technology for Cell Products
46 rdf:type schema:Book
47 Nc1838cbf0b374c7bbf28fe270c13ea44 schema:location Dordrecht
48 schema:name Springer Netherlands
49 rdf:type schema:Organisation
50 Nf5de78a5dad74b56b7607e2d9988c2c3 rdf:first sg:person.01261504775.94
51 rdf:rest rdf:nil
52 Nfcfc70a72e5b44a7ac4b3ca9fef0beef schema:name doi
53 schema:value 10.1007/978-1-4020-5476-1_9
54 rdf:type schema:PropertyValue
55 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
56 schema:name Medical and Health Sciences
57 rdf:type schema:DefinedTerm
58 anzsrc-for:1108 schema:inDefinedTermSet anzsrc-for:
59 schema:name Medical Microbiology
60 rdf:type schema:DefinedTerm
61 sg:person.01034422411.10 schema:affiliation https://www.grid.ac/institutes/grid.418123.d
62 schema:familyName Bollati-Fogolín
63 schema:givenName Mariela
64 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01034422411.10
65 rdf:type schema:Person
66 sg:person.01261504775.94 schema:affiliation https://www.grid.ac/institutes/grid.418123.d
67 schema:familyName Müller
68 schema:givenName Werner
69 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01261504775.94
70 rdf:type schema:Person
71 https://doi.org/10.1002/gene.1030 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018671134
72 rdf:type schema:CreativeWork
73 https://doi.org/10.1126/science.7660125 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062648331
74 rdf:type schema:CreativeWork
75 https://www.grid.ac/institutes/grid.418123.d schema:alternateName GBF German Research Centre for Biotechnology
76 schema:name Department of Experimental Immunology, German Research Centre for Biotechnology (GBF), Braunschweig, Germany
77 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...