Ontology type: schema:Chapter
2007
AUTHORSIngrida Majore , M. Morris Hosseini , Hubert Mayer , Volker Jäger
ABSTRACTFor the generation of human bone tissue in vitro, we were searching for suitable methods to control the ongoing differentiation process of osteogenic cells with a possibility to switch between the expansion of immature progenitor cells and the differentiation into cells of the mature osteogenic phenotype producing mineralised extracellular matrix. This study is focussing on the influence of different basal media formulations which were tested using primary human cells derived from either sites of heterotopic ossification or trabecular bone. The different basal media formulations DMEM, αMEM, RPMI 1640 and our in-house formulation ZKT-I revealed a substantial impact on cellular growth and differentiation. Cells in DMEM were growing relatively slow and differentiated rapidly into mature osteoblasts with strong matrix mineralisation, whereas cells in αMEM were growing more rapidly with slightly slower differentiation and mineralisation kinetics. A strong mineralisation was always accompanied by apoptotic processes as indicated by higher levels of LDH in the supernatant. In contrast, cells in ZKT-I medium showed a sufficient growth with markedly retarded differentiation kinetics which made it most suitable for cell expansion. To induce cell-induced matrix mineralisation using ZKT-I, a moderate increase of the calcium concentration was shown to be a sufficient trigger. With this simple supplementation, ZKT-I proved to be highly suitable for both phases of bone tissue generation in vitro. More... »
PAGES347-351
Cell Technology for Cell Products
ISBN978-1-4020-5475-4
http://scigraph.springernature.com/pub.10.1007/978-1-4020-5476-1_57
DOIhttp://dx.doi.org/10.1007/978-1-4020-5476-1_57
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