Ews-Fli1 in Ewing's Sarcoma: Real Targets and Collateral Damage View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2006

AUTHORS

J. Ban , C. Siligan , M. Kreppel , D. Aryee , H. Kovar

ABSTRACT

Ewing's sarcoma family of tumors (ESFT) are a clinically and scientifically very demanding group of tumors in children and young adults with still unknown histogenesis. The rate-limiting oncogenic mutation in this disease has been identified as a chromosomal translocation, t(11;22)(q24;q12), that leads to the expression of a chimeric transcription factor, EWS-FLI1. We have studied the downstream pathway of EWS-FLI1 by a dual strategy including the isolation of direct target genes from ESFT chromatin and the monitoring of transcriptomic changes after silencing of EWS-FLI1 by RNA interference. This study has lead to the identification of several directly EWS-FLI1-regulated genes and the characterization of their genomic distribution. By comparing several ESFT cell lines, not only variation in overall gene expression patterns downstream of EWS-FLIl was observed, but also differential regulation of directly EWS-FLI1-bound genes. Interestingly, there was variation between members of the same functional gene families. Studies on CD99, another diagnostic hallmark of ESFT, in relation to EWS-FLI1 provided additional evidence for context dependence of fusion protein function. Together, our study represents a first approach to the separation of essential molecular consequences from noise generated by the EWS-FLI1 gene rearrangement in ESFT. More... »

PAGES

41-52

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4020-5133-3_4

DOI

http://dx.doi.org/10.1007/978-1-4020-5133-3_4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1030492340

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/17163154


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Bone Neoplasms", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression Regulation, Neoplastic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Oncogene Proteins, Fusion", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Proto-Oncogene Protein c-fli-1", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "RNA-Binding Protein EWS", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sarcoma, Ewing", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Translocation, Genetic", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Children's Cancer Research Institute, Vienna, Austria", 
          "id": "http://www.grid.ac/institutes/grid.416346.2", 
          "name": [
            "Children's Cancer Research Institute, Vienna, Austria"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ban", 
        "givenName": "J.", 
        "id": "sg:person.01315467366.00", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01315467366.00"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Children's Cancer Research Institute, Vienna, Austria", 
          "id": "http://www.grid.ac/institutes/grid.416346.2", 
          "name": [
            "Children's Cancer Research Institute, Vienna, Austria"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Siligan", 
        "givenName": "C.", 
        "id": "sg:person.0764650651.30", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0764650651.30"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Children's Cancer Research Institute, Vienna, Austria", 
          "id": "http://www.grid.ac/institutes/grid.416346.2", 
          "name": [
            "Children's Cancer Research Institute, Vienna, Austria"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kreppel", 
        "givenName": "M.", 
        "id": "sg:person.01302424500.67", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01302424500.67"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Children's Cancer Research Institute, Vienna, Austria", 
          "id": "http://www.grid.ac/institutes/grid.416346.2", 
          "name": [
            "Children's Cancer Research Institute, Vienna, Austria"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Aryee", 
        "givenName": "D.", 
        "id": "sg:person.015406057707.62", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015406057707.62"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Children's Cancer Research Institute, Vienna, Austria", 
          "id": "http://www.grid.ac/institutes/grid.416346.2", 
          "name": [
            "Children's Cancer Research Institute, Vienna, Austria"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kovar", 
        "givenName": "H.", 
        "id": "sg:person.0741362200.35", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0741362200.35"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "2006", 
    "datePublishedReg": "2006-01-01", 
    "description": "Ewing's sarcoma family of tumors (ESFT) are a clinically and scientifically very demanding group of tumors in children and young adults with still unknown histogenesis. The rate-limiting oncogenic mutation in this disease has been identified as a chromosomal translocation, t(11;22)(q24;q12), that leads to the expression of a chimeric transcription factor, EWS-FLI1. We have studied the downstream pathway of EWS-FLI1 by a dual strategy including the isolation of direct target genes from ESFT chromatin and the monitoring of transcriptomic changes after silencing of EWS-FLI1 by RNA interference. This study has lead to the identification of several directly EWS-FLI1-regulated genes and the characterization of their genomic distribution. By comparing several ESFT cell lines, not only variation in overall gene expression patterns downstream of EWS-FLIl was observed, but also differential regulation of directly EWS-FLI1-bound genes. Interestingly, there was variation between members of the same functional gene families. Studies on CD99, another diagnostic hallmark of ESFT, in relation to EWS-FLI1 provided additional evidence for context dependence of fusion protein function. Together, our study represents a first approach to the separation of essential molecular consequences from noise generated by the EWS-FLI1 gene rearrangement in ESFT.", 
    "editor": [
      {
        "familyName": "Llombart-Bosch", 
        "givenName": "Antonio", 
        "type": "Person"
      }, 
      {
        "familyName": "Felipo", 
        "givenName": "Vicente", 
        "type": "Person"
      }, 
      {
        "familyName": "L\u00f3pez-Guerrero", 
        "givenName": "Jos\u00e9 Antonio", 
        "type": "Person"
      }
    ], 
    "genre": "chapter", 
    "id": "sg:pub.10.1007/978-1-4020-5133-3_4", 
    "isAccessibleForFree": false, 
    "isPartOf": {
      "isbn": [
        "978-1-4020-4966-8", 
        "978-1-4020-5133-3"
      ], 
      "name": "New trends in cancer for the 21st century", 
      "type": "Book"
    }, 
    "keywords": [
      "EWS-FLI1", 
      "overall gene expression patterns", 
      "functional gene families", 
      "fusion protein function", 
      "chimeric transcription factor", 
      "gene expression patterns", 
      "direct target gene", 
      "ESFT cell lines", 
      "genomic distribution", 
      "gene family", 
      "protein function", 
      "transcription factors", 
      "transcriptomic changes", 
      "RNA interference", 
      "target genes", 
      "molecular consequences", 
      "expression patterns", 
      "downstream pathways", 
      "differential regulation", 
      "oncogenic mutations", 
      "chromosomal translocations", 
      "genes", 
      "cell lines", 
      "context dependence", 
      "gene rearrangements", 
      "chromatin", 
      "family", 
      "translocation", 
      "only variation", 
      "mutations", 
      "sarcoma family", 
      "pathway", 
      "regulation", 
      "additional evidence", 
      "dual strategy", 
      "expression", 
      "hallmark", 
      "rearrangement", 
      "variation", 
      "Ewing's sarcoma family", 
      "diagnostic hallmark", 
      "isolation", 
      "members", 
      "target", 
      "identification", 
      "ESFT", 
      "lines", 
      "characterization", 
      "collateral damage", 
      "patterns", 
      "function", 
      "Ewing's sarcoma", 
      "damage", 
      "study", 
      "consequences", 
      "tumors", 
      "evidence", 
      "histogenesis", 
      "factors", 
      "changes", 
      "demanding group", 
      "disease", 
      "distribution", 
      "interference", 
      "strategies", 
      "CD99", 
      "sarcoma", 
      "first approach", 
      "group", 
      "adults", 
      "approach", 
      "unknown histogenesis", 
      "separation", 
      "relation", 
      "monitoring", 
      "dependence", 
      "real target", 
      "young adults", 
      "children", 
      "noise"
    ], 
    "name": "Ews-Fli1 in Ewing's Sarcoma: Real Targets and Collateral Damage", 
    "pagination": "41-52", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1030492340"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/978-1-4020-5133-3_4"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "17163154"
        ]
      }
    ], 
    "publisher": {
      "name": "Springer Nature", 
      "type": "Organisation"
    }, 
    "sameAs": [
      "https://doi.org/10.1007/978-1-4020-5133-3_4", 
      "https://app.dimensions.ai/details/publication/pub.1030492340"
    ], 
    "sdDataset": "chapters", 
    "sdDatePublished": "2022-08-04T17:22", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220804/entities/gbq_results/chapter/chapter_63.jsonl", 
    "type": "Chapter", 
    "url": "https://doi.org/10.1007/978-1-4020-5133-3_4"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/978-1-4020-5133-3_4'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/978-1-4020-5133-3_4'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/978-1-4020-5133-3_4'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/978-1-4020-5133-3_4'


 

This table displays all metadata directly associated to this object as RDF triples.

209 TRIPLES      22 PREDICATES      113 URIs      107 LITERALS      16 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/978-1-4020-5133-3_4 schema:about N1226c007b4684d588437e05c54fd34f6
2 N3226eaa4a7ed43f7a701f9ed7e975812
3 N37e481d5f2f24fa8bb8a9908454bca72
4 N4780d08b000e485ca25eac36c316d7cc
5 N6ffb8d5e38854bbcbb7defad7c740418
6 N7b7521748ef24e4bb3f6c41bc6b95d61
7 N8c05812419bb4c17b4e201c9cc18862f
8 Nbd2c386d74044de4bc9a3cb53266c281
9 anzsrc-for:11
10 schema:author N7c4a0d7e60ca43fdba784d2ad1635d6f
11 schema:datePublished 2006
12 schema:datePublishedReg 2006-01-01
13 schema:description Ewing's sarcoma family of tumors (ESFT) are a clinically and scientifically very demanding group of tumors in children and young adults with still unknown histogenesis. The rate-limiting oncogenic mutation in this disease has been identified as a chromosomal translocation, t(11;22)(q24;q12), that leads to the expression of a chimeric transcription factor, EWS-FLI1. We have studied the downstream pathway of EWS-FLI1 by a dual strategy including the isolation of direct target genes from ESFT chromatin and the monitoring of transcriptomic changes after silencing of EWS-FLI1 by RNA interference. This study has lead to the identification of several directly EWS-FLI1-regulated genes and the characterization of their genomic distribution. By comparing several ESFT cell lines, not only variation in overall gene expression patterns downstream of EWS-FLIl was observed, but also differential regulation of directly EWS-FLI1-bound genes. Interestingly, there was variation between members of the same functional gene families. Studies on CD99, another diagnostic hallmark of ESFT, in relation to EWS-FLI1 provided additional evidence for context dependence of fusion protein function. Together, our study represents a first approach to the separation of essential molecular consequences from noise generated by the EWS-FLI1 gene rearrangement in ESFT.
14 schema:editor N4af34ee11f6849de97cf04be1a368cd6
15 schema:genre chapter
16 schema:isAccessibleForFree false
17 schema:isPartOf Ncaa2869a0d11492e8caec11b9b7838c0
18 schema:keywords CD99
19 ESFT
20 ESFT cell lines
21 EWS-FLI1
22 Ewing's sarcoma
23 Ewing's sarcoma family
24 RNA interference
25 additional evidence
26 adults
27 approach
28 cell lines
29 changes
30 characterization
31 children
32 chimeric transcription factor
33 chromatin
34 chromosomal translocations
35 collateral damage
36 consequences
37 context dependence
38 damage
39 demanding group
40 dependence
41 diagnostic hallmark
42 differential regulation
43 direct target gene
44 disease
45 distribution
46 downstream pathways
47 dual strategy
48 evidence
49 expression
50 expression patterns
51 factors
52 family
53 first approach
54 function
55 functional gene families
56 fusion protein function
57 gene expression patterns
58 gene family
59 gene rearrangements
60 genes
61 genomic distribution
62 group
63 hallmark
64 histogenesis
65 identification
66 interference
67 isolation
68 lines
69 members
70 molecular consequences
71 monitoring
72 mutations
73 noise
74 oncogenic mutations
75 only variation
76 overall gene expression patterns
77 pathway
78 patterns
79 protein function
80 real target
81 rearrangement
82 regulation
83 relation
84 sarcoma
85 sarcoma family
86 separation
87 strategies
88 study
89 target
90 target genes
91 transcription factors
92 transcriptomic changes
93 translocation
94 tumors
95 unknown histogenesis
96 variation
97 young adults
98 schema:name Ews-Fli1 in Ewing's Sarcoma: Real Targets and Collateral Damage
99 schema:pagination 41-52
100 schema:productId N1c0df08c9f66444990a5d44aa1585b40
101 N5a1cfdc5bdcd4986be97c249dfd32511
102 Nc210ce200a6a4b2eb62752dc4b453029
103 schema:publisher N61f43089a999447b8230e481844e552f
104 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030492340
105 https://doi.org/10.1007/978-1-4020-5133-3_4
106 schema:sdDatePublished 2022-08-04T17:22
107 schema:sdLicense https://scigraph.springernature.com/explorer/license/
108 schema:sdPublisher N463a7e57d660433fb59896168bc55bce
109 schema:url https://doi.org/10.1007/978-1-4020-5133-3_4
110 sgo:license sg:explorer/license/
111 sgo:sdDataset chapters
112 rdf:type schema:Chapter
113 N0072f94df855466ea8d5539c3140ba19 rdf:first sg:person.01302424500.67
114 rdf:rest N9039946ab9de40dcbb03f94f6b5d94e8
115 N0dc939f470e645168a2e4c643085003c rdf:first N0fabe166a9174ca792c6087574db05e2
116 rdf:rest rdf:nil
117 N0fabe166a9174ca792c6087574db05e2 schema:familyName López-Guerrero
118 schema:givenName José Antonio
119 rdf:type schema:Person
120 N11205e14c05e4d8ba2a0514efb7d153f rdf:first sg:person.0764650651.30
121 rdf:rest N0072f94df855466ea8d5539c3140ba19
122 N1226c007b4684d588437e05c54fd34f6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
123 schema:name RNA-Binding Protein EWS
124 rdf:type schema:DefinedTerm
125 N1c0df08c9f66444990a5d44aa1585b40 schema:name pubmed_id
126 schema:value 17163154
127 rdf:type schema:PropertyValue
128 N2341ec898a414aaba85a543e492c953e schema:familyName Felipo
129 schema:givenName Vicente
130 rdf:type schema:Person
131 N3226eaa4a7ed43f7a701f9ed7e975812 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
132 schema:name Translocation, Genetic
133 rdf:type schema:DefinedTerm
134 N37e481d5f2f24fa8bb8a9908454bca72 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
135 schema:name Gene Expression Regulation, Neoplastic
136 rdf:type schema:DefinedTerm
137 N463a7e57d660433fb59896168bc55bce schema:name Springer Nature - SN SciGraph project
138 rdf:type schema:Organization
139 N4780d08b000e485ca25eac36c316d7cc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
140 schema:name Humans
141 rdf:type schema:DefinedTerm
142 N4af34ee11f6849de97cf04be1a368cd6 rdf:first Nba57faf6e10b4d8c8eb7f47fe30e74fa
143 rdf:rest Nad39b22edb6049b980117fbe1a837cc1
144 N5a1cfdc5bdcd4986be97c249dfd32511 schema:name doi
145 schema:value 10.1007/978-1-4020-5133-3_4
146 rdf:type schema:PropertyValue
147 N61f43089a999447b8230e481844e552f schema:name Springer Nature
148 rdf:type schema:Organisation
149 N6ffb8d5e38854bbcbb7defad7c740418 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
150 schema:name Oncogene Proteins, Fusion
151 rdf:type schema:DefinedTerm
152 N7b7521748ef24e4bb3f6c41bc6b95d61 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
153 schema:name Bone Neoplasms
154 rdf:type schema:DefinedTerm
155 N7c4a0d7e60ca43fdba784d2ad1635d6f rdf:first sg:person.01315467366.00
156 rdf:rest N11205e14c05e4d8ba2a0514efb7d153f
157 N8bc540f16280438e88abaf512a09236a rdf:first sg:person.0741362200.35
158 rdf:rest rdf:nil
159 N8c05812419bb4c17b4e201c9cc18862f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
160 schema:name Sarcoma, Ewing
161 rdf:type schema:DefinedTerm
162 N9039946ab9de40dcbb03f94f6b5d94e8 rdf:first sg:person.015406057707.62
163 rdf:rest N8bc540f16280438e88abaf512a09236a
164 Nad39b22edb6049b980117fbe1a837cc1 rdf:first N2341ec898a414aaba85a543e492c953e
165 rdf:rest N0dc939f470e645168a2e4c643085003c
166 Nba57faf6e10b4d8c8eb7f47fe30e74fa schema:familyName Llombart-Bosch
167 schema:givenName Antonio
168 rdf:type schema:Person
169 Nbd2c386d74044de4bc9a3cb53266c281 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
170 schema:name Proto-Oncogene Protein c-fli-1
171 rdf:type schema:DefinedTerm
172 Nc210ce200a6a4b2eb62752dc4b453029 schema:name dimensions_id
173 schema:value pub.1030492340
174 rdf:type schema:PropertyValue
175 Ncaa2869a0d11492e8caec11b9b7838c0 schema:isbn 978-1-4020-4966-8
176 978-1-4020-5133-3
177 schema:name New trends in cancer for the 21st century
178 rdf:type schema:Book
179 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
180 schema:name Medical and Health Sciences
181 rdf:type schema:DefinedTerm
182 sg:person.01302424500.67 schema:affiliation grid-institutes:grid.416346.2
183 schema:familyName Kreppel
184 schema:givenName M.
185 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01302424500.67
186 rdf:type schema:Person
187 sg:person.01315467366.00 schema:affiliation grid-institutes:grid.416346.2
188 schema:familyName Ban
189 schema:givenName J.
190 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01315467366.00
191 rdf:type schema:Person
192 sg:person.015406057707.62 schema:affiliation grid-institutes:grid.416346.2
193 schema:familyName Aryee
194 schema:givenName D.
195 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015406057707.62
196 rdf:type schema:Person
197 sg:person.0741362200.35 schema:affiliation grid-institutes:grid.416346.2
198 schema:familyName Kovar
199 schema:givenName H.
200 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0741362200.35
201 rdf:type schema:Person
202 sg:person.0764650651.30 schema:affiliation grid-institutes:grid.416346.2
203 schema:familyName Siligan
204 schema:givenName C.
205 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0764650651.30
206 rdf:type schema:Person
207 grid-institutes:grid.416346.2 schema:alternateName Children's Cancer Research Institute, Vienna, Austria
208 schema:name Children's Cancer Research Institute, Vienna, Austria
209 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...