Ontology type: schema:Chapter
2006
AUTHORSJ. Ban , C. Siligan , M. Kreppel , D. Aryee , H. Kovar
ABSTRACTEwing's sarcoma family of tumors (ESFT) are a clinically and scientifically very demanding group of tumors in children and young adults with still unknown histogenesis. The rate-limiting oncogenic mutation in this disease has been identified as a chromosomal translocation, t(11;22)(q24;q12), that leads to the expression of a chimeric transcription factor, EWS-FLI1. We have studied the downstream pathway of EWS-FLI1 by a dual strategy including the isolation of direct target genes from ESFT chromatin and the monitoring of transcriptomic changes after silencing of EWS-FLI1 by RNA interference. This study has lead to the identification of several directly EWS-FLI1-regulated genes and the characterization of their genomic distribution. By comparing several ESFT cell lines, not only variation in overall gene expression patterns downstream of EWS-FLIl was observed, but also differential regulation of directly EWS-FLI1-bound genes. Interestingly, there was variation between members of the same functional gene families. Studies on CD99, another diagnostic hallmark of ESFT, in relation to EWS-FLI1 provided additional evidence for context dependence of fusion protein function. Together, our study represents a first approach to the separation of essential molecular consequences from noise generated by the EWS-FLI1 gene rearrangement in ESFT. More... »
PAGES41-52
New trends in cancer for the 21st century
ISBN
978-1-4020-4966-8
978-1-4020-5133-3
http://scigraph.springernature.com/pub.10.1007/978-1-4020-5133-3_4
DOIhttp://dx.doi.org/10.1007/978-1-4020-5133-3_4
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1030492340
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/17163154
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