Rice Protoplast Isolation and Transfection for Transient Gene Expression Analysis View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2021-01-21

AUTHORS

Jennylyn L. Trinidad , Toshisangba Longkumer , Ajay Kohli

ABSTRACT

Protoplasts are a versatile and powerful cell-based system to study different plant processes in vivo, due to their ability to maintain cell identity and carry out reactions and metabolic processes similar to intact plants. In rice, despite numerous reports, difficulties are encountered in protoplast isolation and transfection. These include insufficient numbers of protoplasts isolated and inefficient transfection. Such difficulties limit the use of this simple yet useful technology. The need to use protoplasts is particularly important when similar experiments may not work in yeast or Pichia, due to differences in functionally essential protein post-translation modifications. In this chapter, we describe a rice protoplast isolation and transfection method. More... »

PAGES

313-324

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-0716-1068-8_21

DOI

http://dx.doi.org/10.1007/978-1-0716-1068-8_21

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1134714947

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/33471341


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Transfer Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Oryza", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plant Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protoplasts", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transfection", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Strategic Innovation Platform, International Rice Research Institute, Manila, Philippines", 
          "id": "http://www.grid.ac/institutes/grid.419387.0", 
          "name": [
            "Strategic Innovation Platform, International Rice Research Institute, Manila, Philippines"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Trinidad", 
        "givenName": "Jennylyn L.", 
        "id": "sg:person.07402170471.66", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07402170471.66"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan", 
          "id": "http://www.grid.ac/institutes/grid.506932.b", 
          "name": [
            "Strategic Innovation Platform, International Rice Research Institute, Manila, Philippines", 
            "Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Longkumer", 
        "givenName": "Toshisangba", 
        "id": "sg:person.01275722130.26", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01275722130.26"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Strategic Innovation Platform, International Rice Research Institute, Manila, Philippines", 
          "id": "http://www.grid.ac/institutes/grid.419387.0", 
          "name": [
            "Strategic Innovation Platform, International Rice Research Institute, Manila, Philippines"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kohli", 
        "givenName": "Ajay", 
        "id": "sg:person.01276631521.54", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01276631521.54"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "2021-01-21", 
    "datePublishedReg": "2021-01-21", 
    "description": "Protoplasts are a versatile and powerful cell-based system to study different plant processes in vivo, due to their ability to maintain cell identity and carry out reactions and metabolic processes similar to intact plants. In rice, despite numerous reports, difficulties are encountered in protoplast isolation and transfection. These include insufficient numbers of protoplasts isolated and inefficient transfection. Such difficulties limit the use of this simple yet useful technology. The need to use protoplasts is particularly important when similar experiments may not work in yeast or Pichia, due to differences in functionally essential protein post-translation modifications. In this chapter, we describe a rice protoplast isolation and transfection method.", 
    "editor": [
      {
        "familyName": "Bandyopadhyay", 
        "givenName": "Anindya", 
        "type": "Person"
      }, 
      {
        "familyName": "Thilmony", 
        "givenName": "Roger", 
        "type": "Person"
      }
    ], 
    "genre": "chapter", 
    "id": "sg:pub.10.1007/978-1-0716-1068-8_21", 
    "isAccessibleForFree": false, 
    "isPartOf": {
      "isbn": [
        "978-1-0716-1067-1", 
        "978-1-0716-1068-8"
      ], 
      "name": "Rice Genome Engineering and Gene Editing", 
      "type": "Book"
    }, 
    "keywords": [
      "protoplast isolation", 
      "transient gene expression analysis", 
      "protein post-translation modifications", 
      "different plant processes", 
      "post-translation modifications", 
      "gene expression analysis", 
      "cell identity", 
      "cell-based systems", 
      "intact plants", 
      "expression analysis", 
      "plant processes", 
      "metabolic processes", 
      "protoplasts", 
      "transfection method", 
      "transfection", 
      "inefficient transfection", 
      "isolation", 
      "yeast", 
      "plants", 
      "Pichia", 
      "rice", 
      "numerous reports", 
      "similar experiments", 
      "vivo", 
      "identity", 
      "modification", 
      "ability", 
      "process", 
      "insufficient number", 
      "useful technology", 
      "analysis", 
      "number", 
      "experiments", 
      "chapter", 
      "differences", 
      "report", 
      "system", 
      "reaction", 
      "use", 
      "technology", 
      "need", 
      "method", 
      "difficulties", 
      "such difficulties"
    ], 
    "name": "Rice Protoplast Isolation and Transfection for Transient Gene Expression Analysis", 
    "pagination": "313-324", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1134714947"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/978-1-0716-1068-8_21"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "33471341"
        ]
      }
    ], 
    "publisher": {
      "name": "Springer Nature", 
      "type": "Organisation"
    }, 
    "sameAs": [
      "https://doi.org/10.1007/978-1-0716-1068-8_21", 
      "https://app.dimensions.ai/details/publication/pub.1134714947"
    ], 
    "sdDataset": "chapters", 
    "sdDatePublished": "2022-12-01T06:54", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20221201/entities/gbq_results/chapter/chapter_474.jsonl", 
    "type": "Chapter", 
    "url": "https://doi.org/10.1007/978-1-0716-1068-8_21"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/978-1-0716-1068-8_21'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/978-1-0716-1068-8_21'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/978-1-0716-1068-8_21'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/978-1-0716-1068-8_21'


 

This table displays all metadata directly associated to this object as RDF triples.

150 TRIPLES      22 PREDICATES      73 URIs      66 LITERALS      13 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/978-1-0716-1068-8_21 schema:about N32a2f76c7b774b968949682e9e284c95
2 N5fd0aba1e83542068a53c1e497813a86
3 Nd95893761246420583000a385b0c5b65
4 Ndb25593203404e77803eea57184a631b
5 Nf7dee776c3aa4055905c48302d778752
6 anzsrc-for:06
7 anzsrc-for:0601
8 schema:author N6e09a5abd20b431f9413586c20f04a8c
9 schema:datePublished 2021-01-21
10 schema:datePublishedReg 2021-01-21
11 schema:description Protoplasts are a versatile and powerful cell-based system to study different plant processes in vivo, due to their ability to maintain cell identity and carry out reactions and metabolic processes similar to intact plants. In rice, despite numerous reports, difficulties are encountered in protoplast isolation and transfection. These include insufficient numbers of protoplasts isolated and inefficient transfection. Such difficulties limit the use of this simple yet useful technology. The need to use protoplasts is particularly important when similar experiments may not work in yeast or Pichia, due to differences in functionally essential protein post-translation modifications. In this chapter, we describe a rice protoplast isolation and transfection method.
12 schema:editor N9d1d55f6cf3e4d54b831b30b2efac22e
13 schema:genre chapter
14 schema:isAccessibleForFree false
15 schema:isPartOf N7430126a1b1a42a3810721995d86df6d
16 schema:keywords Pichia
17 ability
18 analysis
19 cell identity
20 cell-based systems
21 chapter
22 differences
23 different plant processes
24 difficulties
25 experiments
26 expression analysis
27 gene expression analysis
28 identity
29 inefficient transfection
30 insufficient number
31 intact plants
32 isolation
33 metabolic processes
34 method
35 modification
36 need
37 number
38 numerous reports
39 plant processes
40 plants
41 post-translation modifications
42 process
43 protein post-translation modifications
44 protoplast isolation
45 protoplasts
46 reaction
47 report
48 rice
49 similar experiments
50 such difficulties
51 system
52 technology
53 transfection
54 transfection method
55 transient gene expression analysis
56 use
57 useful technology
58 vivo
59 yeast
60 schema:name Rice Protoplast Isolation and Transfection for Transient Gene Expression Analysis
61 schema:pagination 313-324
62 schema:productId N3d12187ce2a34d29b58e56c45f70990a
63 N466ad527331e484fa32b481c04c615f2
64 Ncee6f5f4b7874c38b419cff2905b25ca
65 schema:publisher Ne0fb6c5edd6c4ca2bec4ac7c329081c8
66 schema:sameAs https://app.dimensions.ai/details/publication/pub.1134714947
67 https://doi.org/10.1007/978-1-0716-1068-8_21
68 schema:sdDatePublished 2022-12-01T06:54
69 schema:sdLicense https://scigraph.springernature.com/explorer/license/
70 schema:sdPublisher Nd26994a9e7ff40b8a17a02cbb9539a76
71 schema:url https://doi.org/10.1007/978-1-0716-1068-8_21
72 sgo:license sg:explorer/license/
73 sgo:sdDataset chapters
74 rdf:type schema:Chapter
75 N32a2f76c7b774b968949682e9e284c95 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
76 schema:name Protoplasts
77 rdf:type schema:DefinedTerm
78 N3d12187ce2a34d29b58e56c45f70990a schema:name doi
79 schema:value 10.1007/978-1-0716-1068-8_21
80 rdf:type schema:PropertyValue
81 N466ad527331e484fa32b481c04c615f2 schema:name pubmed_id
82 schema:value 33471341
83 rdf:type schema:PropertyValue
84 N5e43967b09234687a6e614b62b0e1bca rdf:first N66a978d444474002b596ee7f46ea7d4d
85 rdf:rest rdf:nil
86 N5fd0aba1e83542068a53c1e497813a86 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
87 schema:name Plant Proteins
88 rdf:type schema:DefinedTerm
89 N66a978d444474002b596ee7f46ea7d4d schema:familyName Thilmony
90 schema:givenName Roger
91 rdf:type schema:Person
92 N6e09a5abd20b431f9413586c20f04a8c rdf:first sg:person.07402170471.66
93 rdf:rest Ndf6e66cae8df4ea38707a2edb067fa09
94 N7430126a1b1a42a3810721995d86df6d schema:isbn 978-1-0716-1067-1
95 978-1-0716-1068-8
96 schema:name Rice Genome Engineering and Gene Editing
97 rdf:type schema:Book
98 N9d1d55f6cf3e4d54b831b30b2efac22e rdf:first Nfe5f4d09fda544178ec520c03054a5eb
99 rdf:rest N5e43967b09234687a6e614b62b0e1bca
100 Ncee6f5f4b7874c38b419cff2905b25ca schema:name dimensions_id
101 schema:value pub.1134714947
102 rdf:type schema:PropertyValue
103 Nd26994a9e7ff40b8a17a02cbb9539a76 schema:name Springer Nature - SN SciGraph project
104 rdf:type schema:Organization
105 Nd95893761246420583000a385b0c5b65 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
106 schema:name Oryza
107 rdf:type schema:DefinedTerm
108 Ndb25593203404e77803eea57184a631b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
109 schema:name Gene Transfer Techniques
110 rdf:type schema:DefinedTerm
111 Ndf6e66cae8df4ea38707a2edb067fa09 rdf:first sg:person.01275722130.26
112 rdf:rest Nf07e5733609746fba7f583d47adc2dea
113 Ne0fb6c5edd6c4ca2bec4ac7c329081c8 schema:name Springer Nature
114 rdf:type schema:Organisation
115 Nf07e5733609746fba7f583d47adc2dea rdf:first sg:person.01276631521.54
116 rdf:rest rdf:nil
117 Nf7dee776c3aa4055905c48302d778752 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Transfection
119 rdf:type schema:DefinedTerm
120 Nfe5f4d09fda544178ec520c03054a5eb schema:familyName Bandyopadhyay
121 schema:givenName Anindya
122 rdf:type schema:Person
123 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
124 schema:name Biological Sciences
125 rdf:type schema:DefinedTerm
126 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
127 schema:name Biochemistry and Cell Biology
128 rdf:type schema:DefinedTerm
129 sg:person.01275722130.26 schema:affiliation grid-institutes:grid.506932.b
130 schema:familyName Longkumer
131 schema:givenName Toshisangba
132 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01275722130.26
133 rdf:type schema:Person
134 sg:person.01276631521.54 schema:affiliation grid-institutes:grid.419387.0
135 schema:familyName Kohli
136 schema:givenName Ajay
137 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01276631521.54
138 rdf:type schema:Person
139 sg:person.07402170471.66 schema:affiliation grid-institutes:grid.419387.0
140 schema:familyName Trinidad
141 schema:givenName Jennylyn L.
142 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07402170471.66
143 rdf:type schema:Person
144 grid-institutes:grid.419387.0 schema:alternateName Strategic Innovation Platform, International Rice Research Institute, Manila, Philippines
145 schema:name Strategic Innovation Platform, International Rice Research Institute, Manila, Philippines
146 rdf:type schema:Organization
147 grid-institutes:grid.506932.b schema:alternateName Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan
148 schema:name Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan
149 Strategic Innovation Platform, International Rice Research Institute, Manila, Philippines
150 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...