Molecular Approaches to Diagnosis in Ewing Sarcoma: RT-PCR View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2020-12-17

AUTHORS

Carlos Rodríguez-Martín , Javier Alonso

ABSTRACT

Ewing sarcoma is a rare and aggressive tumor that affects children and young adults. Ewing sarcomas are characterized by specific chromosomal translocations that give rise to fusion transcripts that codify for aberrant transcription factors. More than 95% of Ewing sarcoma harbor translocations that produce the fusion of the EWSR1 gene with the transcription factors FLI1 or ERG. This feature can be used to diagnose this entity unambiguously.In this chapter we describe a RT-PCRReverse transcriptase-PCR (RT-PCR) method that allows for the detection of the most frequent alterations with elevated specificity and sensitivity which is able to distinguish among the different types of fusions. The method is fast and economical, and can be carried out with the conventional equipment available in any molecular biology laboratory. More... »

PAGES

85-103

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-0716-1020-6_7

DOI

http://dx.doi.org/10.1007/978-1-0716-1020-6_7

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1133568612

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/33326095


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