Sheep Erythrocyte Preparation for Hemolytic Tests Exploring Complement Functional Activities View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2021-04-14

AUTHORS

Melchior Chabannes , Pauline Bordereau , Paula Vieira Martins , Marie-Agnès Dragon-Durey

ABSTRACT

Sheep erythrocytes (SE) are commonly used in complement functional tests. Non sensitized SE are useful to study the FH activity of cell protection. Indeed, as the cell surface of sheep erythrocytes is rich in sialic acids, Factor H (FH) is able to bind on it and therefore they represent a model of nonactivating surface. Because of their high capacity of complement regulation SE need to be modified to explore other functionality of the complement pathways, like the Complement hemolytic 50 (CH50) or the AP C3 convertase decay assays. For these tests, SE are sensitized with an anti-sheep red blood cell stroma antibody. In presence of serum or plasma complement components, sensitized SE may initiate complement cascade activation via the classic pathway explored in the CH50 assay. Sensitized SE may also be used to prepare C3b-coated SE that, with the use of buffers favoring AP, are suitable for the C3 Nef hemolytic assay and for the hemolytic assay studying the AP decay activity of FH. In this chapter we describe how to prepare SE for these different hemolytic tests. More... »

PAGES

61-67

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-0716-1016-9_6

DOI

http://dx.doi.org/10.1007/978-1-0716-1016-9_6

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1137163905

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/33847931


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