Cellular Migration Assay: An In Vitro Technique to Simulate the Wound Repair Mechanism View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2020-08-18

AUTHORS

A K M Nawshad Hossian , George Mattheolabakis

ABSTRACT

Wound regeneration is a complex process, which necessitates proper coordination among the inflammatory response, vascularization, matrix formation, and reformation of epithelial tissue. It is a unique process, where healing and regeneration take place simultaneously. Matrix formation is the first critical stage that starts the communication between the keratinocytes, fibroblasts, and integrins. This, in turn, stimulates the differentiation of monocytes into macrophages, to produce cytokines for fibroblasts. This phenomenon is the crucial part for the keratinocyte migration and epithelialization to fill the wound. To understand the complex procedure of wound regeneration, there is a need for easy, convenient, and low-cost approaches that will simulate the wound-repairing process. Scratch assay or cellular migration assay is one of the most convenient and affordable approaches, commonly used by the scientific community. In this chapter, we present the fundamental principles of the experimental procedures required for the Scratch assay. More... »

PAGES

77-83

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-0716-0845-6_8

DOI

http://dx.doi.org/10.1007/978-1-0716-0845-6_8

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1130163697

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/32808260


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