Fluorescent Nanoparticle for Bacteria and DNA Detection View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2007

AUTHORS

Wenjun Zhao , Lin Wang , Weihong Tan

ABSTRACT

Using bioconjugated dye-doped silica nanoparticles (NPs), we have developed a bioassay for the accurate determination of a single bacterial cell within 20 minutes without any signal amplification or sample enrichment. The antibody-conjugated NPs can specifically and quantitatively detect bacteria, such as Escherichia coli O157∶H7 from beef through antibody-antigen recognition. Dye-doped silica NPs have also been successfully used for DNA detection at sub-fentomolar concentrations. Our results demonstrate the potential of dye-doped silica NPs for broad applications in practical biotechnological and medical applications in various biodetection systems. The ultimate goal of integrating bionanotechnology into complex biological systems will emerge as a revolutionary tool for ultrasensitive detection of disease markers and infectious agents. More... »

PAGES

129-135

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-0-387-76713-0_10

DOI

http://dx.doi.org/10.1007/978-0-387-76713-0_10

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1049858509

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/18217340


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