Step-by-Step Protocol for Superparamagnetic Nanoparticle-Based Plasma Membrane Isolation from Eukaryotic Cell. View Full Text


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Chapter Info

DATE

2019

AUTHORS

Deepak B Thimiri Govinda Raj , Niamat Ali Khan , Srisaran Venkatachalam , Dinh Toi Chu

ABSTRACT

Here, we elaborate our detailed protocol for synthesis, functionalization, and application of superparamagnetic nanoparticle (SPMNP) for plasma membrane and lysosome isolation. We used standard thermal decomposition-based synthesis of iron oxide (Fe3O4) core SPMNP 1.0. Using ligand addition methodology, we surface functionalized SPMNP 1.0 with phospholipids and generated phospholipid-SPMNP 2.0. Further we used NH2-phospholipid-SPMNP 2.0 to isolate plasma membrane. Using our SPMNP subcellular fractionation protocol, we are able to isolate high-pure-high-yield plasma membrane using NH2-phospholipid-SPMNP 2.0. As a future perspective, we propose to use SPMNP on clinical patient samples and perform mass spectrometry-based proteomics, lipidomics, and glycomics for early cancer diagnosis. More... »

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/7651_2019_211

DOI

http://dx.doi.org/10.1007/7651_2019_211

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1112157128

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30771189


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