Alveolar Rhabdomyosarcoma Decellularization View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2017-05-25

AUTHORS

Michela Pozzobon , Mattia Saggioro , Stefania D’Agostino , Gianni Bisogno , Maurizio Muraca , Piergiorgio Gamba

ABSTRACT

In cancer research, it is an urgent need in the obtainment of a simple and reproducible model that mimics in all the complexity the pathological microenvironment. Specifically, the will to improve the overall survival of young patients affected by rhabdomyosarcoma compels researchers to develop new models resembling the multifaceted environment of the pathology to deeply study the disease under novel and different aspects. To this end, we developed a decellularization protocol for alveolar rhabdomyosarcoma (ARMS) able to maintain the three-dimensional structure. The attained extracellular matrix (ECM) can be used as 3D in vitro model suitable to both recapitulate the in vivo cancer microenvironment, and also for drug testing. Here, we first describe a detergent-enzymatic method and then we analyze the decellularization efficiency and the scaffold proteins. More... »

PAGES

317-325

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/7651_2017_45

DOI

http://dx.doi.org/10.1007/7651_2017_45

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1085588969

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28540560


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