Isolation and Expansion of Muscle Precursor Cells from Human Skeletal Muscle Biopsies View Full Text


Ontology type: schema:Chapter      Open Access: True


Chapter Info

DATE

2016-04-01

AUTHORS

Chiara Franzin , Martina Piccoli , Luca Urbani , Carlo Biz , Piergiorgio Gamba , Paolo De Coppi , Michela Pozzobon

ABSTRACT

One of the major issues concerning human skeletal muscle progenitor cells is represented by the efficient isolation and in vitro expansion of cells retaining the ability to proliferate, migrate and differentiate once transplanted. Here we describe a method (1) effective in obtaining human muscle precursor cells both from fresh and frozen biopsies coming from different muscles, (2) selective to yield cells uniformly positive for CD56 and negative for CD34 without FACS sorting, (3) reliable in maintaining proliferative and in vitro differentiative capacity up to passage 10. More... »

PAGES

195-204

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/7651_2016_321

DOI

http://dx.doi.org/10.1007/7651_2016_321

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1008609762

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/27032940


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