Imaging Pluripotency: Time-Lapse Analysis of Mouse Embryonic Stem Cells View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2015-07-11

AUTHORS

Anna Pezzarossa , Ana M. V. Guedes , Domingos Henrique , Elsa Abranches

ABSTRACT

The current view of the pluripotent state is that of a transient, dynamic state, maintained by the balance between opposing cues. Understanding how this dynamic state is established in pluripotent cells and how it relates to gene expression is essential to obtain a more detailed description of the pluripotent state.In this chapter, we describe how to study the dynamic expression of a core pluripotency gene regulator—Nanog—by exploiting single-cell time-lapse imaging of a reporter mESC line grown in different cell culture media. We further describe an automated image analysis method and discuss how to extract information from the generated quantitative time-course data. More... »

PAGES

87-100

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/7651_2015_255

DOI

http://dx.doi.org/10.1007/7651_2015_255

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1010501178

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/26162772


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