A PCR-based Protocol for In Vitro Selection of Non-crosshybridizing Oligonucleotides View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2003-01-16

AUTHORS

Russell Deaton , Junghuei Chen , Hong Bi , Max Garzon , Harvey Rubin , David Harlan Wood

ABSTRACT

DNA computing often requires oligonucleotides that do not produce erroneous cross-hybridizations. By using in vitro evolution, huge libraries of non-crosshybridizing oligonucleotides might be evolved in the test tube. As a first step, a fitness function that corresponds to non-crosshybridization has to be implemented in an experimental protocol. Therefore, a modified version of PCR that selects non-crosshybridizing oligonucleotides was designed and tested. Experiments confirmed that the PCR-based protocol did amplify maximally mismatched oligonucleotides selectively over those that were more closely matched. In addition, a reaction temperature window was identified in which discrimination between matched and mismatched might be obtained. These results are a first step toward practical manufacture of very large libraries of non-crosshybridizing oligonucleotides in the test tube. More... »

PAGES

196-204

Book

TITLE

DNA Computing

ISBN

978-3-540-00531-5
978-3-540-36440-5

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/3-540-36440-4_17

DOI

http://dx.doi.org/10.1007/3-540-36440-4_17

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1007945416


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