Characterization of Non-crosshybridizing DNA Oligonucleotides Manufactured In Vitro View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2005

AUTHORS

Junghuei Chen , Russell Deaton , Max H. Garzon , Jin Woo Kim , David Wood , Hong Bi , Dylan Carpenter , Yu-Zhen Wang

ABSTRACT

Libraries of DNA oligonucleotides manufactured by an In Vitro selection protocol were characterized for their non-crosshybridizing properties. Cloning and sequencing after several iterations of the protocol showed that the sequences, in general, became more non-crosshybridizing. Gel electrophoresis of protocol product, also, indicated non-crosshybridization, and showed evolution in the population of molecules under the non-crosshybridization selection pressure. Melting curves of protocol product also indicated non-crosshybridization when compared to control samples. Thus, it appears that the protocol does select populations of non-crosshybridizing sequences. More... »

PAGES

50-61

Book

TITLE

DNA Computing

ISBN

978-3-540-26174-2
978-3-540-31844-6

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/11493785_5

DOI

http://dx.doi.org/10.1007/11493785_5

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1033473398


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