A Proteomic Approach to the Molecular Analysis of Kindling View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2005

AUTHORS

John T. Slevin , Sidney W. Whiteheart , Thomas C. Vanaman

ABSTRACT

Application of the newly developed tools of proteomics analysis to the brain is still at an early stage of development. Primary application of 2D gel electrophoresis coupled with mass spectrometry based protein identification has been primarily directed at analyzing whole brain proteomes. As has been recently reviewed,1 this has led to the identification of a number of the more abundant brain proteins but has yet to focus on the important functional sub-compartments of the neuron (e.g., the synapse) or to probe for potential changes in disease states. This, in part, reflects a major limitation of protein profiling: the inherent complexity of proteomes even of subcellular compartments. We have begun to establish 2D gel maps for rat hippocampal synaptosomes and sub-fractions (synaptosol, plasma membrane and vesicle fractions) in both the resting and depolarized state. In this chapter we demonstrate how this technique can be applied to the study of kindled epilepsy. More... »

PAGES

189-197

References to SciGraph publications

Book

TITLE

Kindling 6

ISBN

978-0-387-24380-1

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/0-387-26144-3_19

DOI

http://dx.doi.org/10.1007/0-387-26144-3_19

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1023929487


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