Identification of Altered Glycosylation as the Major Difference Between Intracellulary Accumulated and Secreted β-Trace Protein Produced in Baculovirus-Infected Insect Cells View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2002-01-01

AUTHORS

Gryssell Rodriguez , Harald S. Conradt , Volker Jäger

ABSTRACT

ConclusionsSDS-PAGE/western blotting of protein from supernatant as well as from lysed virus-infected insect cells (IPLB SF21 AE, BTI Tn5Bl-4) revealed the presence of an intracellularly acumulated β-TP species, which was demonstrated to have a molecular weight different to those observed in the supernatant.Enzymatic digestion with N-Glycosidase F has proved that the difference between the intra-and extracellular protein pattern was due to different glycosylation stages of the recombinant protein.The results suggest the presence of a bottle neck in the glycosylation pathway of baculovirus-infected insect cells secreting β-trace protein. More... »

PAGES

153-155

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/0-306-46860-3_27

DOI

http://dx.doi.org/10.1007/0-306-46860-3_27

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1042178122


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