Method For Determining Homologous Recombination Repair Activity


Ontology type: sgo:Patent     


Patent Info

DATE

2019-07-04T00:00

AUTHORS

YOSHINO YUKI , CHIBA NATSUKO , ENDO SHINO

ABSTRACT

A method for measuring homologous recombination repair activity in cells, including: cutting a double strand of a target genome DNA region inside a cell at a specific site by using a site-specific nuclease; introducing a donor vector including a tag sequence to the cell and, by homologous recombination, incorporating the marker sequence at the specific site of the cut in the double strand in the target genome DNA region; and measuring a fused DNA sequence including the target genome DNA region in the cell and the marker sequence. More... »

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