Methods, Systems And Devices For Selection And Generation Of Genome Edited Clones


Ontology type: sgo:Patent     


Patent Info

DATE

N/A

AUTHORS

LAVIEU, GREGORY G. , MOCCIARO, Annamaria , GUAN, Xiao , MCEWEN, Jason M. , SOUMILLON, Magali , NEVILL, J. TANNER , KURZ, VOLKER L. , DYCK, Patricia A.

ABSTRACT

Methods are described herein for isolating clonal populations of cells having a defined genetic modification. The methods are performed, at least in part, in a microfluidic device comprising one or more sequestration pens. The methods include the steps of: maintaining individual cells (or precursors thereof) that have undergone a genomic editing process in corresponding sequestration pens of a microfluidic device; expanding the individual cells into respective clonal populations of cells; and detecting, in one or more cells of each clonal population, the presence of a first nucleic acid sequence that is indicative of the presence of an on-target genome edit in the clonal population of cells. Also described are methods of performing genome editing within a microfluidic device, and compositions comprising one or more clonal populations of cells generated according to the methods disclosed herein. More... »

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