Methods And Compositions For Genomic Target Enrichment And Selective Dna Sequencing


Ontology type: sgo:Patent     


Patent Info

DATE

N/A

AUTHORS

LIZARDI, PAUL M. , FERGUSON, BRENT W.

ABSTRACT

It has been established that one or more large double stranded DNA fragments (each 2,000 to 40,000 base pairs in size) can be captured and isolated from genomic DNA fragments using sequence specific PNA hybridization probes. Compositions and methods for enrichment of a multiplicity of long DNA sequences selected from the genome of any eukaryote are provided. Capture is performed using multiple PNA molecules with gamma-modified chiral backbones, comprising a mixture of neutral and positive chemical groups such as diethylene glycol, gamma-L-lysine, gamma-L-thialysine. Two or more PNA probes with covalently bound haptens, preferably biotin, target each DNA domain of interest for capture, isolation, and subsequent sequencing analysis of the multiplicity of enriched targets, including DNA methylation sequencing. The methods include enhancement of probe-DNA binding specificity through single strand binding proteins (SSB). More... »

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