Functional Genomics Using Crispr-Cas Systems For Saturating Mutagenesis Of Non-Coding Elements, Compositions, Methods, Libraries And Applications Thereof


Ontology type: sgo:Patent     


Patent Info

DATE

N/A

AUTHORS

BAUER, Daniel, E. , ORKIN, STUART, H. , SANJANA, Neville, Espi , SHALEM, Ophir , WRIGHT, JASON , ZHANG, FENG

ABSTRACT

The application relates to a deep scanning mutagenesis library to interrogate phenotypi c changes in a population of cells comprising a plurality of CRISPR-Cas system guide RNAs targeting genomic sequences within at least one continuous genomic region, wherein the guide RNAs target at least 100 genomic sequences upstream of a PAM sequence for every 1000 base pairs within the continuous genomic region and methods for their use. More... »

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