Methods For Quantitating Dna Using Digital Multiple Displacement Amplification


Ontology type: sgo:Patent     


Patent Info

DATE

2014-09-25T00:00

AUTHORS

BLAINEY PAUL , XU LIYI

ABSTRACT

The present invention provides improved methods for detecting contamination in WGA reagents as well as improved methods for quantitating nucleic acids, such as DNA. The present invention relates to novel methods of quantifying nucleic acids involving whole-genome amplification (WGA) reaction components and a dye molecule to detects nucleic acids and partitioning reactions to quantify nucleic acids. The present application relates in particular to the use of digital multiple displacement amplification (dMDA) performed in microfluidic droplets or in hydrogel matrices for quantifying nucleic acids from a sample, e.g. contaminant nucleic acids in MDA reagent. More... »

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