Methods Of In Vivo Engineering Of Large Sequences Using Multiple Crispr/Cas Selections Of Recombineering Events


Ontology type: sgo:Patent     


Patent Info

DATE

N/A

AUTHORS

CARSTENS, CARSTEN-PETER

ABSTRACT

The present invention provides a method for making a large nucleic acid having a defined sequence in vivo. The method combines recombineering techniques with a CRISPR/Cas system to permit multiple insertions of defined sequences into a target nucleic acid at one time, double stranded cleavage of target nucleic acids in which the defined sequences were not successfully inserted, and selection of successful recombinant cells. The method further includes repeating the process one or more times, using a successful recombinant from one round as the host cell for the next round. More... »

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